肌桥缝接神经处的bFGF复合膜对神经再生的影响
作者：张庭琛 安贵林 苏秋香 辛畅泰 李崇杰 郑桂芬
单位：张庭琛 安贵林 苏秋香(沈阳医学院组织胚胎学教研室，沈阳 110031)；辛畅泰 李崇杰 郑桂芬(沈阳医学院附属中心医院手外科)
关键词：肌桥；碱性成纤维细胞生长因子；神经再生；兔
解剖科学进展000320摘 要：目的，探讨bFGF复合膜对肌桥桥接神经后的再生效果的影响。方法：在自体肌桥缝接缺损神经处置入bFGF复合膜并通过组织学和图像分析等方法对兔正中神经缺损后再生的组织结构进行比较观察。结果：实验侧再生神经纤维的数量及平均直径均明显大于对照侧，具有显著性差异。与对照侧比较，实验则再生神经纤维的髓鞘较厚，着色较深。实验侧桥接段再生神经纤维间的胶原纤维比对照侧的少。结论；在自体肌桥缝接神经处置入bFGF复合膜，可以提高肌桥桥接缺损神经的再生效果。
The Effect of Compound Membrane Containing bFGF on the Regeneration of Defect nerve in Muscular Bridge
, 百拇医药
Zhang Tingchen ,Xin Changtai ,An Guilin
(Department of Histo-Embryology, Shenyang Medical College, Shenyang, 110031)
Abstract：Objective: To explore the effect of the compound membrane containing bFGF on regeneration of defect nerve in muscle bridge. Method: The regeneration of the defect nerve is observed by the methods of histology and image-analysis, after the median nerve of rabbits were cut off and sewed with muscle bridge which was surrounded by compound membrane containing bFGF. Results: Amount of regenerative nerve fibers in the experimental group is more than the one in the control group (P<0.05). The myelin sheath of regenerative nerve fibers in the experimental group is thicker and deeper after staining with osmate than the one in the control group. Amount of collagenous fiber among the regenerative nerve fibers in the experimental group is less than the one in the control group. Conclusion: The compound membrane containing bFGF could promote the regeneration of defect nerve in the muscle bridge.
, 百拇医药
Keywords：muscle bridge; bFGF; nerve regeneration; rabbit
参考文献：
[1]Ide C. Nerve regeneration through the basal lamina scaffold of the skeletal muscle. Neumsci Res, 1984, 8(1): 379
[2]keynes KT, Hopkin WG, Huang LH, et al. Regeneration of mouse peripheral nerve in degenerating skeletal muscle. Guidance by the residual muscle fiber basement membrane. Brain Res, 1984, 295(2): 275
, http://www.100md.com
[3]钟世震.骨骼肌桥接神经缺损研究的进展.中国临床解剖学杂志，1989，7(1)：51
[4]梁启善，魏善新，林电.血管和雪旺氏细胞植入变性骨骼肌修复周围神经缺损的实验研究.实用手外科杂志，1995，9(1)：16
[5]辛畅泰，安贵林，张庭琛，等.不同骨骼肌桥接动物神经缺损的实验研究，中华手外科杂志，1996，12(4)：249
[6]孔吉明，钟世震，孙博，等.用骨骼肌桥接周围神经缺损的实验研究.中华显微外科杂志，1986，9(2)：68
[7]邵宁生，王会信，周廷冲.成纤维细胞生长因子对神经元的影响作用.生理科学进展，1994，25(1)：17
[8]黄巨恩，陈维平.成纤维细胞生长因子的基础与应用研究.解剖学进展，1996，2(2)：193
[9]唐孝明，裴福兴，谭建三.成纤维细胞生长因子促进神经再生的实验研究.中华显微外科杂志，1998，21(3)：201
[10]利春叶，曹代成.成纤维细胞生长因子促进坐骨神经损伤后功能的恢复.中华显微外科杂志，1998，2l(4)：274, http://www.100md.com
单位：张庭琛 安贵林 苏秋香(沈阳医学院组织胚胎学教研室，沈阳 110031)；辛畅泰 李崇杰 郑桂芬(沈阳医学院附属中心医院手外科)
关键词：肌桥；碱性成纤维细胞生长因子；神经再生；兔
解剖科学进展000320摘 要：目的，探讨bFGF复合膜对肌桥桥接神经后的再生效果的影响。方法：在自体肌桥缝接缺损神经处置入bFGF复合膜并通过组织学和图像分析等方法对兔正中神经缺损后再生的组织结构进行比较观察。结果：实验侧再生神经纤维的数量及平均直径均明显大于对照侧，具有显著性差异。与对照侧比较，实验则再生神经纤维的髓鞘较厚，着色较深。实验侧桥接段再生神经纤维间的胶原纤维比对照侧的少。结论；在自体肌桥缝接神经处置入bFGF复合膜，可以提高肌桥桥接缺损神经的再生效果。
The Effect of Compound Membrane Containing bFGF on the Regeneration of Defect nerve in Muscular Bridge
, 百拇医药
Zhang Tingchen ,Xin Changtai ,An Guilin
(Department of Histo-Embryology, Shenyang Medical College, Shenyang, 110031)
Abstract：Objective: To explore the effect of the compound membrane containing bFGF on regeneration of defect nerve in muscle bridge. Method: The regeneration of the defect nerve is observed by the methods of histology and image-analysis, after the median nerve of rabbits were cut off and sewed with muscle bridge which was surrounded by compound membrane containing bFGF. Results: Amount of regenerative nerve fibers in the experimental group is more than the one in the control group (P<0.05). The myelin sheath of regenerative nerve fibers in the experimental group is thicker and deeper after staining with osmate than the one in the control group. Amount of collagenous fiber among the regenerative nerve fibers in the experimental group is less than the one in the control group. Conclusion: The compound membrane containing bFGF could promote the regeneration of defect nerve in the muscle bridge.
, 百拇医药
Keywords：muscle bridge; bFGF; nerve regeneration; rabbit
参考文献：
[1]Ide C. Nerve regeneration through the basal lamina scaffold of the skeletal muscle. Neumsci Res, 1984, 8(1): 379
[2]keynes KT, Hopkin WG, Huang LH, et al. Regeneration of mouse peripheral nerve in degenerating skeletal muscle. Guidance by the residual muscle fiber basement membrane. Brain Res, 1984, 295(2): 275
, http://www.100md.com
[3]钟世震.骨骼肌桥接神经缺损研究的进展.中国临床解剖学杂志，1989，7(1)：51
[4]梁启善，魏善新，林电.血管和雪旺氏细胞植入变性骨骼肌修复周围神经缺损的实验研究.实用手外科杂志，1995，9(1)：16
[5]辛畅泰，安贵林，张庭琛，等.不同骨骼肌桥接动物神经缺损的实验研究，中华手外科杂志，1996，12(4)：249
[6]孔吉明，钟世震，孙博，等.用骨骼肌桥接周围神经缺损的实验研究.中华显微外科杂志，1986，9(2)：68
[7]邵宁生，王会信，周廷冲.成纤维细胞生长因子对神经元的影响作用.生理科学进展，1994，25(1)：17
[8]黄巨恩，陈维平.成纤维细胞生长因子的基础与应用研究.解剖学进展，1996，2(2)：193
[9]唐孝明，裴福兴，谭建三.成纤维细胞生长因子促进神经再生的实验研究.中华显微外科杂志，1998，21(3)：201
[10]利春叶，曹代成.成纤维细胞生长因子促进坐骨神经损伤后功能的恢复.中华显微外科杂志，1998，2l(4)：274, http://www.100md.com