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TGFα-PE40对人肺腺癌细胞生长的抑制作用
http://www.100md.com 《癌症》2000年第2期
TGFα-PE40|肺腺癌细胞|表皮生长因子受体|生长抑制剂
     作者:王兵 徐永华 王杰军 黄渊 江万里 陆健 高勇

    单位:王兵(第二军医大学长征医院肿瘤科,上海 2000032);徐永华(中国科学院上海细胞生物学研究所,上海 2000313);王杰军(第二军医大学长征医院肿瘤科,上海 2000032);黄渊(上海市第一人民医院附属护校,上海 2000804);江万里(中国科学院上海细胞生物学研究所,上海 2000313);陆健(第二军医大学卫勤系医学统计学教研室,上海 200433);高勇(第二军医大学长征医院肿瘤科,上海 2000032)

    关键词:TGFα-PE40;肺腺癌细胞;表皮生长因子受体;生长抑制剂

    癌症000213

    【摘 要】目的:探讨基因重组转化生长因子α-绿脓杆菌外毒素融合蛋白(TGFα-PE40,简称TP40)对人肺腺癌细胞生长的导向抑制作用。方法:用免疫组化LSAB法检测人肺腺癌SPC-A-1细胞表皮生长因子受体(EGFR)的表达,用结晶紫染色法和3H-亮氨酸掺入法检测TP40对SPC-A-1细胞生长及蛋白质合成的抑制,用过量EGF竞争拮抗TP40的细胞毒作用。结果:SPC-A-1细胞免疫组化显示出大量棕黄色的EGFR阳性染色。TP40浓度为1~100ng/ml时,对SPC-A-1细胞生长及蛋白质合成呈剂量依赖性抑制。过量EGF能完全拮抗TP40的细胞毒作用。结论:人肺腺癌SPC-A-1细胞能高水平地表达EGFR,重组TP40对SPC-A-1细胞生长具有较强的抑制作用,作用部位为细胞的EGFR。

    中图分类号:R734.2;R73-36 文献标识码:A

    文章编号:1000-467X(2000)02-0143-03

    Growth inhibitory effect of TGFα-PE40 on human lung adenocarcinoma cells

    WANG Bing XU Yong-hua WANG Jie-jun et al

    (Department of Oncology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,P.R.China)

    【Abstract】 Objective: To investigate growth inhibit effect of recombinant transforming growth factor α-Pseudomonas exotoxin fusion protein (TGFα-PE40;TP40) on cultured human lung adenocarcinoma cells. Methods: The expression of epidermal growth factor receptor (EGFR) in a human lung adenocarcinoma cell line SPC-A-1 was determined with immunohistochemistry. Inhibitory effects of TP40 on SPC-A-1 cell growth and protein synthesis were analyzed with crystal violet staining and 3H-leucine incorporation. Competitive assay was performed by the addition of excess of EGF. Results:The SPC-A-1 cells appeared large amount of brown immunoperoxidase reaction indicating EGFR positive. When the concentration of TP40 was in the range of 1~ 100 ng/ml, TP40 inhibited SPC-A-1 cell growth and protein synthesis in a dose-dependent manner. Excess EGF can completely block the inhibitory effects of TP40. Conclusions:The human lung adenocarcinoma SPC-A-1 cells express high level EGFR. TP40 can significantly inhibit SPC-A-1 cell growth. The cytotoxic effect of TP40 was specifically mediated by EGFR. ......

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