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间期FISH在鼻咽癌细胞系及石腊切片中的应用初探
http://www.100md.com 《癌症》1999年第4期
间期|荧光原位杂交|鼻咽肿瘤|石蜡切片
     作者:谢鹭,周文,许亮国,杨旭宇,姚开泰

    单位:湖南医科大学肿瘤研究所(长沙,410078)

    关键词:间期;荧光原位杂交;鼻咽肿瘤;石蜡切片

    New Page 1

    【摘要】目的:建立间期荧光原位杂交技术,检测鼻咽癌细胞系和鼻咽癌石蜡切片中9号和3号染色体的数目改变情况。方法:中期染色体片及细胞系滴片制备,间期及石蜡切片荧光原位杂交。结果:9号染色体在鼻咽癌细胞系中为六倍体,在石蜡包埋鼻咽癌组织中也呈多倍体改变。3号染色体在鼻咽癌细胞系中呈三倍体改变,在石蜡包埋鼻咽癌组织中则无非整倍性改变。结论:间期荧光原位杂交为一很有应用前景的技术,可以用于存档石蜡块的研究,考察染色体数目和/或特异基因改变情况,有一定诊断学意义,值得推广应用。

    中图分类号:R739.6 文献标识码:A 文章编号:1000-467X(1999)04-0364-04

    The primary applications of interphase FISH on nasophayngeal carcinoma cell lineand paraffin-embeddedsections

    XIE Lu,ZHOU Wen,XU Liang-guo,et al.

    Cancer Research Institute, Hunan Medical University, Chang sha 410078, P.R.China

    【Abstract】Objective:To build up interphase FISH technique, to detect chromosome 9 and chromosome 3 copy numbers in nasopharyngeal carcinoma (NPC) cell lines and paraffin-embedded sections. Methods:Preparation of slide spreads of metaphase chrosomes and interphase cell lines, interphase and paraffin section fluorescence in situ hybridization (FISH). Result:Chromosome 9 showed hectasomy in NPC cell lines, showed multisomy change too in paraffin-embedded NPC tissue. Chromosome 3 showed trisomy in NPC cell lines, but copy munber is normal in NPC tissue. Conclusion: Interphase FISH is a technique of wonderful application perspective, may be used in archival paraffin-embedded tissues to detect chromosome copy changes and/or specific gene amplification or deletion, may be helpful in tumor diagnostics, and is worth of wide use. ......

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