用简并寡核苷酸探针筛选对虾白斑综合征病毒DNA多聚酶基因片段
作者：石正丽 Durand Stephanie Bonami Jean-Robert
单位：石正丽(中国科学院武汉病毒研究所，武汉 430071；UMR 219， UMII，CNRS/IFREMER， CC-80， Plane E. Bataillon, 34095,Montpellier CEDEX 5, France)；Durand Stephanie Bonami Jean-Robert(UMR 219， UMII，CNRS/IFREMER， CC-80， Plane E. Bataillon, 34095,Montpellier CEDEX 5, France)
关键词：白斑综合征病毒；DNA多聚酶
中国病毒学000316 摘要：根据一些病毒的DNA多聚酶氨基酸序列中特有的保守序列VYGDTD设计的简并寡核苷酸，经地高辛标记后与对虾白斑综合征病毒基因库克隆杂交，筛选出一段长度为707 bp的EcoR I基因片段，该片段在一个开放阅读框内。并含DNA多聚酶B家族特有的保守序列YGDTDS。经与基因库比较，其氨基酸序列与藻类DNA病毒科(Phycodnaviridae)的几株藻类病毒的DNA多聚酶片段有部分相似，因此推测该核苷酸片段为对虾白斑综合征病毒DNA多聚酶基因的部分序列。
, 百拇医药
中图分类号：S945.479 文献标识码：A
文章编号：1003-5125(2000)03-0302-06
Screening of DNA Polymerase Gene from White Spot Syndrome
Virus (WSSV) by Using Degenerated Oligonucleotides
SHI Zheng-li
(Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China；
UMR 219, CNRS/IFREMER, UMII, Place E. Bataillon, 34095 Montpellier, CEDEX5, France)
, http://www.100md.com
Durand Stephanie，Bonami Jean-Robert
(UMR 219, CNRS/IFREMER, UMII, Place E. Bataillon, 34095 Montpellier, CEDEX5, France)
Abstract: Degenerated oligonucleotides were designed from one motif VYGDTDS of several viral DNA polymerases and were labelled with Digoxigenin to screen DNA polymerase gene in a White Spot Syndrome Virus (WSSV) DNA library. One EcoR I fragment of 707 bp was selected and contains a putative open reading frame in which the motif YGDTDS of family B of DNA polymerase was found. This selected sequence was subsequently compared with GenBank data. The results showed that this deduced amino acids sequence has some similarities with several algal-like virus of the family Phycodnavirus. So we deduced that this is one DNA polymerase gene fragment of WSSV.
, http://www.100md.com
Key words: White Spot Syndrome Virus (WSSV); DNA polymerase
作者简介：石正丽(1964年-)，河南省人，女，研究员，博士，主要从事海洋生物的病毒学及分子生物学的研究
参考文献
[1] Bulach DM, Kumar CA, Zaia A, et al. Group Ⅱ nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequence [J]. J Invertr Pathol, 1999,73:59-73
[2] 黄捷，于佳，宋晓玲，等.对虾皮下及造血组织坏死杆状病毒的精细结构，核酸多肽及血清学研究[J].海洋水产研究，1995，16(1)：11-19
, http://www.100md.com
[3] Takahashi Y, Itami, T, Kondo M, et al. Electron microscopy evidence of bacilliform virus infection in Kuruma shrimp (Penaeus japonicus) [J]. Fish Pathol, 1994,29:121-125
[4] Wongteerasupaya C, Vickers JE, Sriuairatana S, et al. A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon [J]. Dis Aquat Org, 1995,21:69-77
[5] Wang CH, Lo CF, Leu JH, et al. Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon [J]. Dis Aquat Org 1995,23:239-242
, http://www.100md.com
[6] Lo CF, Ho CH, Peng SE, et al. White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods [J]. Dis Aquat Org, 1996, 27:215-225
[7] Lightner DV. A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp [M]. Baton Rouge: World Aquaculture Society, 1996
[8] Durand S, Lightner DV, Redman RM, et al. Ultrastructure and morphogenesis of White Spot Syndrome Baculovirus (WSSV) [J] Dis Aquat Org, 1997,29:205-211
, http://www.100md.com
[9] Durand S. Etude d'une baculovirose (WSS) des crevettes penaeides: caracterisation et construction d'outils de diagnostic [D]. Thesis de doctorat, Université Monpellier Ⅱ.1997
[10] Murphy F A, Fauquet CM, Bishop DHL, et al. Virus taxonomy. Archives of Virology, Springer-Verlag, Vienna, 1995,pp586
[11] 石正丽，黄灿华，陈棣华.中国对虾杆状病毒基因克隆及探针制备与检测[J].中国病毒学，1998，13(3)：263-267
[12] Ito J & Braithwaite DK. Compilation and alignment of DNA polymerase sequences [J]. Nucleic Acids Research, 1991,19(15):4045-4057
, 百拇医药
[13] Chen F, Suttle CA, Short SM. Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase genes [J] Appl Environ Mocrobiol, 1996,62(8):2869-2874
[14] Chen F & Suttle CA. Evolutionary relationship among large double-stranded DNA viruses that infect microalgae and other organisms as inferred from DNA polymerase genes [J]. Virology, 1996,219:170-178
收稿日期：1999-12-26，修回日期：2000-04-04, 百拇医药
单位：石正丽(中国科学院武汉病毒研究所，武汉 430071；UMR 219， UMII，CNRS/IFREMER， CC-80， Plane E. Bataillon, 34095,Montpellier CEDEX 5, France)；Durand Stephanie Bonami Jean-Robert(UMR 219， UMII，CNRS/IFREMER， CC-80， Plane E. Bataillon, 34095,Montpellier CEDEX 5, France)
关键词：白斑综合征病毒；DNA多聚酶
中国病毒学000316 摘要：根据一些病毒的DNA多聚酶氨基酸序列中特有的保守序列VYGDTD设计的简并寡核苷酸，经地高辛标记后与对虾白斑综合征病毒基因库克隆杂交，筛选出一段长度为707 bp的EcoR I基因片段，该片段在一个开放阅读框内。并含DNA多聚酶B家族特有的保守序列YGDTDS。经与基因库比较，其氨基酸序列与藻类DNA病毒科(Phycodnaviridae)的几株藻类病毒的DNA多聚酶片段有部分相似，因此推测该核苷酸片段为对虾白斑综合征病毒DNA多聚酶基因的部分序列。
, 百拇医药
中图分类号：S945.479 文献标识码：A
文章编号：1003-5125(2000)03-0302-06
Screening of DNA Polymerase Gene from White Spot Syndrome
Virus (WSSV) by Using Degenerated Oligonucleotides
SHI Zheng-li
(Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China；
UMR 219, CNRS/IFREMER, UMII, Place E. Bataillon, 34095 Montpellier, CEDEX5, France)
, http://www.100md.com
Durand Stephanie，Bonami Jean-Robert
(UMR 219, CNRS/IFREMER, UMII, Place E. Bataillon, 34095 Montpellier, CEDEX5, France)
Abstract: Degenerated oligonucleotides were designed from one motif VYGDTDS of several viral DNA polymerases and were labelled with Digoxigenin to screen DNA polymerase gene in a White Spot Syndrome Virus (WSSV) DNA library. One EcoR I fragment of 707 bp was selected and contains a putative open reading frame in which the motif YGDTDS of family B of DNA polymerase was found. This selected sequence was subsequently compared with GenBank data. The results showed that this deduced amino acids sequence has some similarities with several algal-like virus of the family Phycodnavirus. So we deduced that this is one DNA polymerase gene fragment of WSSV.
, http://www.100md.com
Key words: White Spot Syndrome Virus (WSSV); DNA polymerase
作者简介：石正丽(1964年-)，河南省人，女，研究员，博士，主要从事海洋生物的病毒学及分子生物学的研究
参考文献
[1] Bulach DM, Kumar CA, Zaia A, et al. Group Ⅱ nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequence [J]. J Invertr Pathol, 1999,73:59-73
[2] 黄捷，于佳，宋晓玲，等.对虾皮下及造血组织坏死杆状病毒的精细结构，核酸多肽及血清学研究[J].海洋水产研究，1995，16(1)：11-19
, http://www.100md.com
[3] Takahashi Y, Itami, T, Kondo M, et al. Electron microscopy evidence of bacilliform virus infection in Kuruma shrimp (Penaeus japonicus) [J]. Fish Pathol, 1994,29:121-125
[4] Wongteerasupaya C, Vickers JE, Sriuairatana S, et al. A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon [J]. Dis Aquat Org, 1995,21:69-77
[5] Wang CH, Lo CF, Leu JH, et al. Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon [J]. Dis Aquat Org 1995,23:239-242
, http://www.100md.com
[6] Lo CF, Ho CH, Peng SE, et al. White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods [J]. Dis Aquat Org, 1996, 27:215-225
[7] Lightner DV. A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp [M]. Baton Rouge: World Aquaculture Society, 1996
[8] Durand S, Lightner DV, Redman RM, et al. Ultrastructure and morphogenesis of White Spot Syndrome Baculovirus (WSSV) [J] Dis Aquat Org, 1997,29:205-211
, http://www.100md.com
[9] Durand S. Etude d'une baculovirose (WSS) des crevettes penaeides: caracterisation et construction d'outils de diagnostic [D]. Thesis de doctorat, Université Monpellier Ⅱ.1997
[10] Murphy F A, Fauquet CM, Bishop DHL, et al. Virus taxonomy. Archives of Virology, Springer-Verlag, Vienna, 1995,pp586
[11] 石正丽，黄灿华，陈棣华.中国对虾杆状病毒基因克隆及探针制备与检测[J].中国病毒学，1998，13(3)：263-267
[12] Ito J & Braithwaite DK. Compilation and alignment of DNA polymerase sequences [J]. Nucleic Acids Research, 1991,19(15):4045-4057
, 百拇医药
[13] Chen F, Suttle CA, Short SM. Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase genes [J] Appl Environ Mocrobiol, 1996,62(8):2869-2874
[14] Chen F & Suttle CA. Evolutionary relationship among large double-stranded DNA viruses that infect microalgae and other organisms as inferred from DNA polymerase genes [J]. Virology, 1996,219:170-178
收稿日期：1999-12-26，修回日期：2000-04-04, 百拇医药