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肝细胞癌组织和癌周组织中p53基因密码子249突变的比较研究
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肝肿瘤|p53基因|突变
     作者:彭晓谋,彭文伟,姚春斓,陈雪娟

    单位:

    关键词:肝肿瘤;p53基因;突变

    癌症990511 【摘 要】 目的:本研究通过比较研究HCC癌组织和癌周组织中p53基因密码子249特征性突变来进一步阐明该基因在HCC发生机制中的意义。方法:收集9份癌组织中经单链构型多态性分析研究证实存在密码子249突变的HCC标本和11例无此突变的HCC标本,采用等位基因特异性PCR(AS-PCR)方法比较研究癌组织和癌周组织中密码子249突变情况。结果:20例HCC中,癌周组织中密码子249特征性突变检出率为70%。9例SSCP阳性者的癌周组织中的特征性突变检出率达100%。11例阴性患者的癌组织和癌周组织也能检出一定水平的突变。结论:p53基因密码子249突变在非癌肝组织中广泛存在。癌组织中密码了249特征性突变可能发生在肿瘤形成之前。

    中图分类号:R735.7;R730.231 文献标识码:A 文章编号:1000-467X(1999)05-0526-03

    Comparison of codon 249 mutations of p53 gene in cancerous tissues with that in pericancerous tissues of HCCs

    PENG Xiao-mou, PENG Wen-wei, YAO Chun-lan,et al.

    Viral Hepatitis Laboratory,Sun Yat-sen University of Medical Sciences,Guangzhou 510630,P.R. China

    【Abstract】 Objective:To analyze codon 249 mutation of p53 gene in cancerous tissue and pericancerous tissue of HCC comparatively,and study the significance of p53 gene in hepatocarcinogenesis. Methods:Codon 249 mutations were detected using allele-specific PCR in cancerous and pericancerous tissues of 9 codon 249 mutation positive cases and 11 negative cases detected by single-stranded conformational polymorphism (SSCP) analysis in former research. Results:The detective rate of codon 249 mutation in pericancerous tissues was 70% (14/20). That in 9 cases of SSCP positive HCCs was 100% (9/9).Codon 249 mutations were also detected in cancerous and pericancerous tissues of 11 SSCP negative HCCs.Conclusions:Codon 249 mutations of p53 gene were very popular in non-neoplastic tissues.Those mutations in cancerous tissues might take place in the stage before the formation of tumor. ......

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