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抗癌药物引起胃癌细胞系BGC-823凋亡的作用观察
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     作者:陈维鹏 李东生

    单位:潍坊医学院附属淄博市第一人民医院外科 山东淄博 255200

    关键词:胃癌细胞系;BGC-823;凋亡;丝裂霉素;5-Fu;卡铂

    潍坊医学院000120摘 要:目的观察多种抗癌药物对体外培养胃癌细胞系BGC-823的作用及引起凋亡的机制。方法选用丝裂霉素(MMC)、卡铂、5-Fu、卡铂+5-Fu,作用于呈指数生长的BGC-823胃癌细胞系细胞,在作用24,48h时,分别观察细胞的凋亡情况,利用镜下细胞计数方法,计数细胞凋亡的百分数;并分别留取正常培养12,24h,抗癌药物作用24,48h的培养液上清,测定癌胚抗原(CEA)的含量。结果选用5-Fu、MMC、卡铂3种药物作用于体外培养胃癌细胞系MGC-823细胞,其引起凋亡的结果有较大的差异:5-Fu在48h后仅产生5%的细胞凋亡;作用24hMMC引起的凋亡数为50%,卡铂为25%,卡铂+5-Fu为30%;作用48h后MMC引起的凋亡数为98%,卡铂为80%~85%,卡铂+5-Fu为85%~90%。CEA测定结果:作用前后无明显变化。结论5-Fu对体外培养胃癌细胞系BGC-823的作用不明显;MMC、卡铂对胃癌细胞系BGC-823的作用强大,特别体现在作用48h后;卡铂与5-Fu的协同作用不明显;3种抗癌药物对胃癌细胞系BGC-823产生CEA的抑制作用不明显。
, 百拇医药
    分类号:R735.2;R446.5 文献标识码:A

    Anticancer Drugs and Their Effect to Induce Apoptosis to BGC-823 Gastric Cell Line

    Chen Weipeng ,Li Dongsheng

    (Department of Surgery,Zibo First People's Hospital the Affilanted Hospital of Weifang Medical College,Shandong,255200)

    Abstract:Objective To observe the morphologist of cell apoptoses of BGC-823 gastric cancer cell line after the effecting of anticancer drugs.Method Using mitomicin,carboplatinum,5-Fu,to act to the BGC-823 gastric cancer cell line for 24,48 hours,respectively.Count for the rate of the cell apoptoses,and remain the culturing liquid of which normal cultured and acted for 24,48 hours by anticancer drugs,exam the content of CEA.Result The result of those three kind of anticancer drugs that causes cell apoptoses is different.The rate of apoptoses induced by 5-Fu is 3%after acted for 24 hours;and MMC,is 50%;carboplatinum,25%;carboplatinum+5-Fu,30%.After 48hours,5-Fu,5%;MMC,98%;carboplatinum,80%;carboplatinum+5-Fu,90%,respectively.The consistency change of CEA has no statistic significance.Conclusion 5-Fu has no evident effect to BGC-823;but MMC,carboplatinum has strive effect,especially after 48 hours;the coordination effect of carboplatinum+5-Fu is faint,and the inhibition effect of those three anticancer drugs to the produce of CEA has no statistic significance.
, 百拇医药
    Keywords:Gastric cancer line;BGC-823;Apoptosis;Mitomicin;5-Fu;Carboplatinum

    基金项目:山东省淄博市科委资助课题(批准号94140)

    参考文献: [1]李连弟,鲁凤珠,张思维,等.1990年~1992年恶性肿瘤发病及死亡率[J].中华肿瘤杂志,1996,6:403

    [2]Guido Majio,Isabelle J.Apoptosis,oncosis,and necrosis an overview of cell death[J].Am J Pathol,1995,146(1):3

    [3]Inada T,Ichikawa A,Kubota T,et al.5-Fu induced apoptosis correlates with efficacy against human gastric and colon cancer:xeonografts in nude mice[J].Anticancer Res,1997,17(3c):1965

    [4]Schwartz GK,Haimovitz FA,Dhupar sK,et al.Potentiation of apoptosis by treatment with the protein kinase C-specific inhibitor[J].J Nall Cancer Inst,1995,87(18):1394

    收稿日期:1999-07-21, http://www.100md.com