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编号:10292578
反义bcl-2基因转染对单核白血病细胞存活及化疗耐受能力的影响
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bcl-2|反义核酸|U937细胞系|化疗药物
     作者:何凤田 朱锡华 黄云辉

    单位:何凤田 朱锡华 黄云辉(第三军医大学免疫学教研室,重庆 400038)

    关键词:bcl-2;反义核酸;U937细胞系;化疗药物

    癌症000417

    【摘要】目的:探讨bcl-2反义核酸在单核白血病细胞系U937细胞中的作用,以丰富bcl-2及其反义核酸调控肿瘤细胞凋亡的资料。方法:将反义bcl-2基因表达载体pLXSN/as-bcl-2转染于U937细胞,建立U937/as-bcl-2细胞模型,利用流式细胞技术检测模型细胞bcl-2的表达水平;通过细胞计数观察模型细胞在正常培养条件下和有化疗药物Ara-C或三尖杉酯碱存在时的生长和存活特性变化。结果:①模型细胞的bcl-2蛋白表达水平明显下降。②模型细胞在正常培养条件下的生长和存活表型无明显改变。③在Ara-C或三尖杉酯碱存在的情况下,模型细胞的存活率较对照细胞者明显下降。结论:①单独反义bcl-2基因转染可明显抑制U937细胞bcl-2的表达,但对细胞在正常条件下的生长和存活表型无明显影响。②反义bcl-2基因转染可增加U937细胞对化疗药物的敏感性。

    中图分类号:Q753;Q733.7 文献标识码:A 文章编号:1000-467X(2000)04-0344-03

    Influence of antisense bcl- 2 gene on survival of mononuclear leukemia cell U937 and the sensitivity of the cell to chemotherapeutic drugs

    HE Feng- tian ZHU Xi- hua HUANG Yun- hui

    (Department of Immunology, The Third Military Medical University, Chongqing 400038, P. R. China)

    【Abstract】 Objective: To enrich the data of tumor cell apoptosis regulated by bcl- 2 and antisense bcl- 2 through studying the role of antisense bcl- 2 in mononuclear leukemia cell line U937. Methods: A U937 cell model (U937/as- bcl- 2) was established by transferring the antisense bcl- 2 expression vector pLXSN/as- bc1- 2 into U937 cell. bcl- 2 expression in the model cell was detected by fluorescence- activated cell sorter. The model cell growth and survival were analyzed by counting the numbers of total and survival cells in normal culture condition or in the presence of Ara- C or harringtonine. Results: ① bcl- 2 expression in the model cell significantly decreased. ② The model cell had a normal growth and survival in normal culture condition.③ The percentage of the survival model cells significantly decreased in the presence of Ara- C or harringtonine. Conclusions: ① Antisense bcl- 2 gene transfer alone can significantly suppress bcl- expression in U937 cells, but it had no obvious effect on the cell growth and survival in normal culture condition. ② Antisense bcl- 2 gene transfer can increase the sensitivity of U937 cell to chemotherapeutic drugs. ......

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