电针对力竭游泳大鼠肾脏线粒体自由基代谢和线粒体功能的影响
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(1.河南省体工大队医务所,郑州450003;2.河南中医学院针推系)[摘 要] 目的:进一步研究电针的抗过氧化、抗衰老作用,探讨肾俞平衡阴阳、益精补肾机理。方法:选择7组大鼠做力竭游泳实验,观察力竭前后不同时间及有无电针刺激条件下大鼠肾脏线粒体自由基代谢情况和线粒体功能的变化。结果:发现力竭游泳后即刻和4小时末MDA,GSH,NADH,游离Ca2+,THG,GSHPx均有不同特点、程度的变化;电针组大鼠游泳耐力时间明显长于未电针组(P Methods Changes of metabolism of mitochondrial free radicals and mitochondial function in kidney were investigated at different times after full swimming and electroacupuncture.Results MDA,GSH,NADH,free Ca2+,THG,GSHPx at 0 and 4 hours after swimmimg had changes in varying degrees;the swimming time in the electroacupuncture group was significantly longer than that in the nonelectroacupuncture group.Conclusion Full swimming can induce a series of hyperoxidation,and production,accumalation and abnormal metabolism of a great number of free radicals in rats;Electroacupuncture at Shenshu can obviously increase the ability of antihyperoxidation,effectively clearing free radicals and increasing motor ability,which reveal preliminarily the mechanism of Shenshu in balancing Yin and Yang,supplementing vital essence and tonifying kidney. ......
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