郑 培，汤兵力，林艳珠，冯春艳，刑征宇(海南省食品药品检验所海口分所，海口 570216)

    UPLC-MS/MS法同时测定裸花紫珠干浸膏中5种有效成分的含量

    郑 培*，汤兵力，林艳珠，冯春艳，刑征宇(海南省食品药品检验所海口分所，海口 570216)

    目的：建立同时测定裸花紫珠干浸膏中5种有效成分含量的方法。方法：采用超高效液相色谱-串联质谱法。色谱条件：色谱柱为Zorbax Eclipse Plus C18，流动相为乙腈-水(梯度洗脱)，流速为0.3 mL/min，柱温为40℃，进样量为2 μL；质谱条件：多反应监测离子扫描模式，干燥气/雾化气为氮气，干燥气温度为325℃，干燥气流量为6 L/min，雾化气压力为45 psi，鞘流气温度为350℃，鞘流气流量为12 L/min，毛细管电压为4 000 V(+)、3 500 V(-)，喷嘴电压为500 V。结果：木犀草苷、毛蕊花糖苷、槲皮素、木犀草素、芦丁检测质量浓度线性范围分别为0.504 8～252.4 ng/mL(r＝0.999 9)、0.712 4～356.2 ng/mL(r＝0.999 0)、0.509 4～254.7 ng/mL(r＝0.996 2)、0.303 0～151.5 ng/mL(r＝0.999 8)、0.602 2～301.1 ng/mL(r＝0.999 6)；精密度、稳定性、重复性试验的RSD<3.0%；定量限分别为0.42、0.87、0.33、0.12、0.76 ng/mL；加样回收率分别为97.99%～101.20%(RSD＝1.3%，n＝6)、96.50%～101.20%(RSD＝1.7%，n＝6)、94.81%～99.34%(RSD＝1.7%，n＝6)、97.54%～100.51%(RSD＝1.2%，n＝6)、93.37%～98.70%(RSD＝1.9%，n＝6)。结论：该方法操作简便，精密度、稳定性、重复性好，可用于裸花紫珠干浸膏中5种有效成分含量的同时测定。

    超高效液相色谱-串联质谱法；裸花紫珠干浸膏；木犀草苷；毛蕊花糖苷；槲皮素；木犀草素；芦丁

    ABSTRACTOBJECTIVE：To establish a method for simultaneous determination of 5 effective components in Luohua zizhu dry extract.METHODS：UPLC-MS/MS was conducted.The separation was performed on an Zorbax Eclipse Plus C18column with mobile phase of acetonitrile-water(gradient elution)at the flow rate of 0.3 mL/min.The column temperature was set at 40℃and sample size was 2 μL.The analytes were detected in the multiple reaction monitoring(MRM)mode.Nitrogen was used as drying gas and atomized gas.The temperature and flow rate of drying gas were 325℃and 6 L/min.The pressure of atomized gas was 45 psi. The temperature and flow rate of sheath gas were 350℃and 12 L/min.The voltage of capillary were 4 000 V(+)and 3 500 V(-). The voltage of nozzle was 500 V.RESULTS：The linear ranges of luteoloside ......