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Effect on different doncentrations of exogenous hormones on
baicalin in Scutellaria baicalensis callus
WAN Gui-xiang， MA Lin*， ZHANG Jian
(Tianjin University of Traditional Chiness Medicine， Tianjin 300193， China)
[Abstrct] Objective： To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones. Method： HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions： ODS column was adopted， with methanol-water-phosphate(47∶53∶0.2) as the mobile phase. The flow velocity was 1 mL·min-1， the detective wavelength was 280 nm， and the temperature of column was room temperature.Result： S. baicalensis callus induced by 6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1 showed the highest baicalin content， up to 49.78 mg·g-1. Conclusion： The experiment is such a simple， rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.
[Key words] Scutlaria baicalensis； callus； baicalin； HPLC
doi：10.4268/cjcmm20122424
[责任编辑 孔晶晶], http://www.100md.com(万贵香,马琳,张坚)
Effect on different doncentrations of exogenous hormones on
baicalin in Scutellaria baicalensis callus
WAN Gui-xiang， MA Lin*， ZHANG Jian
(Tianjin University of Traditional Chiness Medicine， Tianjin 300193， China)
[Abstrct] Objective： To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones. Method： HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions： ODS column was adopted， with methanol-water-phosphate(47∶53∶0.2) as the mobile phase. The flow velocity was 1 mL·min-1， the detective wavelength was 280 nm， and the temperature of column was room temperature.Result： S. baicalensis callus induced by 6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1 showed the highest baicalin content， up to 49.78 mg·g-1. Conclusion： The experiment is such a simple， rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.
[Key words] Scutlaria baicalensis； callus； baicalin； HPLC
doi：10.4268/cjcmm20122424
[责任编辑 孔晶晶], http://www.100md.com(万贵香,马琳,张坚)