白木香查尔酮合酶(AsCHS1)基因的克隆和生物信息学分析(4)
Medical College， Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine，Wanning 571533， China； 4. College of Pharmacy， Shandong University of Traditional Chinese Medicine， Ji′nan 250355， China)
[Abstract] Objective： The study aimed to clone the open reading frame of chalcone synthase (CHS) from Aquilaria sinensis and analyze the bioinformatics and expression of the gene. Method： One unique sequence containing CHS domain was discovered in our previous reported wound transcriptome dataset of A. sinensis. The open reading frame of CHS was cloned by RT-PCR strategy with the template of mixed RNA extracted from A. sinensis stem which treated by different wound time.The bioinformatic analysis of this gene and its corresponding protein was performed. The AsCHS1 expression in calli was analyzed with histone gene as an internal control gene under wound condition by qRT-PCR technique. Result： One unique sequence of CHS， named as AsCHS1， was cloned from A. sinensis. The full length of AsCHS1 cDNA was containing a 1 192 bp ORF that encoded 397 amino acids. The result of qRT-PCR displayed that the highest expression level was at 12 h， which indicated that it was possibly involved in early-stage response to wound. Conclusion： Cloning and analyzing AsCHS1 gene from A. sinensis provided basic information for study the fuction and expression regulation of AsCHS1 in the flavonoids biosynthesis .
[Key words] chalcone synthase； Aquilaria sinensis； flavonoids biosynthesis
doi：10.4268/cjcmm20130202
[责任编辑 吕冬梅], 百拇医药(汪孟曦，李文兰，张争，魏建和，杨云，徐艳红，梁良)
[Abstract] Objective： The study aimed to clone the open reading frame of chalcone synthase (CHS) from Aquilaria sinensis and analyze the bioinformatics and expression of the gene. Method： One unique sequence containing CHS domain was discovered in our previous reported wound transcriptome dataset of A. sinensis. The open reading frame of CHS was cloned by RT-PCR strategy with the template of mixed RNA extracted from A. sinensis stem which treated by different wound time.The bioinformatic analysis of this gene and its corresponding protein was performed. The AsCHS1 expression in calli was analyzed with histone gene as an internal control gene under wound condition by qRT-PCR technique. Result： One unique sequence of CHS， named as AsCHS1， was cloned from A. sinensis. The full length of AsCHS1 cDNA was containing a 1 192 bp ORF that encoded 397 amino acids. The result of qRT-PCR displayed that the highest expression level was at 12 h， which indicated that it was possibly involved in early-stage response to wound. Conclusion： Cloning and analyzing AsCHS1 gene from A. sinensis provided basic information for study the fuction and expression regulation of AsCHS1 in the flavonoids biosynthesis .
[Key words] chalcone synthase； Aquilaria sinensis； flavonoids biosynthesis
doi：10.4268/cjcmm20130202
[责任编辑 吕冬梅], 百拇医药(汪孟曦，李文兰，张争，魏建和，杨云，徐艳红，梁良)