冷胁迫下铁皮石斛抗寒相关基因的SCoT差异表达分析(4)
[18]Donald E Nelson， Peter P Repetti， Tom R Adams， et al. Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres[J]. Proc Natl Acad Sci USA， 2007，10(42)：16450.
[19]李慧聪，郭秀林，王冬梅，等.玉米热激蛋白70基因对温度胁迫的响应[J].河北农业大学学报，2010，33(6)：12.
SCoT differential expression of cold resistance related genes in
Dendrobium officinale under low temperature stressLI Dong-bin， GAO Yan-hui， SI Jin-ping
(Nurturing Station for the State Key Laboratory of Subtropical Silviculture， Zhejiang Provinical Strategic Allance for
Technical Innovation in Industry of Dendrobium officinale Zhejiang Agricultural and Forestry University， Lin′an 311300， China)[Abstract]In order to study the molecule mechanism of the differential expression in Dendrobium officinale under low temperature， the high cold resistance germplasms were used for constructing the RNA pools. SCoT markers were used to analyze the different cDNA pools transcribed from the RNA pools. 11 transcripts derived fragments from 500 cDNA amplified bands were amplified by 64 primers， and were sorted out， cloned， sequenced and analyzed. The results showed that cDNA pools with SCoT markers could be used for differential display in D. officinale under low temperature stress. Sequence analysis indicated that the transcripts derived fragments were significantly homologous in nucleotide sequence with membrane-associated proteins， osmotic regulation protein， CBF transcriptional factor， resistance protein. One left gene segments functions were still unknown， which may be related to the cold resistant gene expression in D. officinale.
[Key words]Dendrobium officinale； cold resistance； SCoT； cold resistance gene； differential expression
doi：10.4268/cjcmm20130409[责任编辑吕冬梅], 百拇医药(李东宾 高燕会 斯金平)
[19]李慧聪，郭秀林，王冬梅，等.玉米热激蛋白70基因对温度胁迫的响应[J].河北农业大学学报，2010，33(6)：12.
SCoT differential expression of cold resistance related genes in
Dendrobium officinale under low temperature stressLI Dong-bin， GAO Yan-hui， SI Jin-ping
(Nurturing Station for the State Key Laboratory of Subtropical Silviculture， Zhejiang Provinical Strategic Allance for
Technical Innovation in Industry of Dendrobium officinale Zhejiang Agricultural and Forestry University， Lin′an 311300， China)[Abstract]In order to study the molecule mechanism of the differential expression in Dendrobium officinale under low temperature， the high cold resistance germplasms were used for constructing the RNA pools. SCoT markers were used to analyze the different cDNA pools transcribed from the RNA pools. 11 transcripts derived fragments from 500 cDNA amplified bands were amplified by 64 primers， and were sorted out， cloned， sequenced and analyzed. The results showed that cDNA pools with SCoT markers could be used for differential display in D. officinale under low temperature stress. Sequence analysis indicated that the transcripts derived fragments were significantly homologous in nucleotide sequence with membrane-associated proteins， osmotic regulation protein， CBF transcriptional factor， resistance protein. One left gene segments functions were still unknown， which may be related to the cold resistant gene expression in D. officinale.
[Key words]Dendrobium officinale； cold resistance； SCoT； cold resistance gene； differential expression
doi：10.4268/cjcmm20130409[责任编辑吕冬梅], 百拇医药(李东宾 高燕会 斯金平)