M.DL 2 000 DNA Marker；1～3.Lasiodiplodia theobromae strains of Baimuxiang-YF01，Baimuxiang-YF02，Baimuxiang-YF03；4.阴性对照(水作模板)。

    图2 通用引物ITS1/ITS4对可可毛色二孢的PCR扩增

    Fig.2 Amplification product of DNA from Lasiodiplodia theobromae using primers ITS1 and ITS4

    图3 基于葡萄座腔菌属菌rDNA-ITS区域碱基序列构建的NJ系统发育树

    Fig.3 Phylogenetic tree constructed by the neighbor-joining method based on the sequences of the internal transcribed spacer (ITS)1-5.8S-ITS2 ribosomal DNA regions of Botryosphaeria spp. isolated

    在病部产生明显的小黑粒 ......