噬菌体展示技术与中药活性成分靶点蛋白筛选研究(5)
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Study on phage display technology and target protein screening for
active ingredients of traditional Chinese medicines
ZHANG Fang1， QIU Zheng2*
(1. School of Pharmacy， Nanjing University of Chinese Medicine， Nanjing 210023， China；
2. School of Life Science & Biotechnology， China Pharmaceutical University， Nanjing 210009， China)
[Abstract] Phage display technology refers to a high-throughput in vitro screening technology for extracting required peptides/proteins from colonies with mass mutants. Due to its high efficiency， practicability and convenience， it has been widely applied in pharmaceutical research and development， as well as target protein screening for active ingredients of traditional Chinese medicines. Target protein is the binding site of drug molecules in vivo， and good targets are the basis of excellent pharmaceuticals. This article summarizes the advance in studies on the phage display technology and its application in targeted protein screening for active ingredients of Chinese materia medica.
[Key words] phage display technology； active ingredient of Chinese materia medica； target protein； screening
doi：10.4268/cjcmm20131408, http://www.100md.com(张芳 邱郑)
Study on phage display technology and target protein screening for
active ingredients of traditional Chinese medicines
ZHANG Fang1， QIU Zheng2*
(1. School of Pharmacy， Nanjing University of Chinese Medicine， Nanjing 210023， China；
2. School of Life Science & Biotechnology， China Pharmaceutical University， Nanjing 210009， China)
[Abstract] Phage display technology refers to a high-throughput in vitro screening technology for extracting required peptides/proteins from colonies with mass mutants. Due to its high efficiency， practicability and convenience， it has been widely applied in pharmaceutical research and development， as well as target protein screening for active ingredients of traditional Chinese medicines. Target protein is the binding site of drug molecules in vivo， and good targets are the basis of excellent pharmaceuticals. This article summarizes the advance in studies on the phage display technology and its application in targeted protein screening for active ingredients of Chinese materia medica.
[Key words] phage display technology； active ingredient of Chinese materia medica； target protein； screening
doi：10.4268/cjcmm20131408, http://www.100md.com(张芳 邱郑)