滨蒿内酯和泽兰内酯在云南石斛物种中的分布(4)
[27] 李文莉，汪文涛，雷玉萍，等、 RP-HPLC法测定脉络宁注射液中滨蒿内酯的含量[J]、中草药，2004， 35(7)：762、[28] 周颖，夏厚林，彭颖，等、 滨蒿内酯鉴别迭鞘石斛可行性的探讨[J]、成都中医药大学学报， 2011，34(3)： 51、Study on distributed patterns of scoparone and ayapin in
Dendrobium species from Yunnan
LIU Yan-yan， YU Hong*， YUAN Feng， ZHOU Feng， CHEN Ye-gao
(Yunnan Herbal Laboratory， Institute of Herb Biotic Resources， Yunnan University， Kunming 650091， China)
[Abstract] An HPLC method for determination of scoparone and ayapin was established for investigating the distributed patterns of scoparone and ayapin in 37 species of Dendrobium. The contents of scoparone and ayapin in varied collected samples were determined by the established HPLC method. The pseudo-bulbs sampled were collected according to different growth age of D. thyrsiflorum. The results showed that the contents of scoparone and ayapin were much differently distributed in species of Dendrobium. Only D. thyrsiflorum and D. densiflorum contained both scoparone and ayapin， the content decreased with the growth age. A fewer amount of ayapin was tested in D. loddigesii from Wenshan. The scoparone and ayapin were not determined in the rest species of Dendrobium. The method was concise， sensitive， accurate and reproducible. It could be applied to assay scoparone and ayapin in populations of herbal Dendrobium.
[Key words] HPLC； scoparone； ayapin； Dendrobium
doi：10.4268/cjcmm20132118
[责任编辑 吕冬梅], http://www.100md.com(刘妍妍 虞泓 袁峰 周峰 陈业高)
Dendrobium species from Yunnan
LIU Yan-yan， YU Hong*， YUAN Feng， ZHOU Feng， CHEN Ye-gao
(Yunnan Herbal Laboratory， Institute of Herb Biotic Resources， Yunnan University， Kunming 650091， China)
[Abstract] An HPLC method for determination of scoparone and ayapin was established for investigating the distributed patterns of scoparone and ayapin in 37 species of Dendrobium. The contents of scoparone and ayapin in varied collected samples were determined by the established HPLC method. The pseudo-bulbs sampled were collected according to different growth age of D. thyrsiflorum. The results showed that the contents of scoparone and ayapin were much differently distributed in species of Dendrobium. Only D. thyrsiflorum and D. densiflorum contained both scoparone and ayapin， the content decreased with the growth age. A fewer amount of ayapin was tested in D. loddigesii from Wenshan. The scoparone and ayapin were not determined in the rest species of Dendrobium. The method was concise， sensitive， accurate and reproducible. It could be applied to assay scoparone and ayapin in populations of herbal Dendrobium.
[Key words] HPLC； scoparone； ayapin； Dendrobium
doi：10.4268/cjcmm20132118
[责任编辑 吕冬梅], http://www.100md.com(刘妍妍 虞泓 袁峰 周峰 陈业高)