羌活不同地理种群ITS序列变异及系统发生分析(1)
[摘要]利用核糖体rRNA基因内转录间隔区(ITS)序列，对我国羌活31个野生群体的遗传结构进行了分析。经PCR扩增测序，获得长度为634～635 bp的ITS核苷酸序列，其平均G+C量(578%)显著高于A+T量。在羌活31个居群402条序列中共检测到31个变异位点，多态位点比例为488%，其中，简约信息位点为12个，共有31种单倍型。AMOVA分析结果显示：羌活大部分的遗传变异发生在居群间(57%)。以宽叶羌活为外群构建的NJ树显示：31个单倍型并没有按地理分布形成明显的族群，各地理单元中的单倍型相互混杂，没有明显的地理分化模式。
[关键词]羌活；ITS；序列分析；遗传结构
[Abstract]In this study， 31 Notopterygium incisum populations were analyzed using ITS sequences to investigate the genetic structure.The results showed that： the ITS region ranged in size from 634 to 635 bp and base composition was with high G+C content of 578% Thirtyone polymorphic sites were detected from 402 sequences of 31 populations of N incisum ......
您现在查看是摘要页，全文长 3609 字符。
[关键词]羌活；ITS；序列分析；遗传结构
[Abstract]In this study， 31 Notopterygium incisum populations were analyzed using ITS sequences to investigate the genetic structure.The results showed that： the ITS region ranged in size from 634 to 635 bp and base composition was with high G+C content of 578% Thirtyone polymorphic sites were detected from 402 sequences of 31 populations of N incisum ......
您现在查看是摘要页，全文长 3609 字符。