【摘要】目的 探讨热应激创伤对PC12细胞热休克蛋白70(HSP70)的影响。方法 用免疫细胞化学方法检测热应激对PC12细胞HSP70表达水平的变化。结果 在热损伤过程中HSP70和细胞增殖能力均显著高于对照组。结论 HSP70高水平表达作为是神经细胞自我保护的机制之一。

    【关键词】热休克蛋白70创伤热应激PC12细胞

    【中图分类号】Q753【文献标识码】A 【文章编号】1004-7484(2010)03-00-02

    Effects of heat shock protein 70 in traumic stress of PC 12 cells

    【Abstract】Objective: To investigate the changes of heat shock protein 70 (HSP70) expression levels in neurons in traumic stress. Methods: The expression amounts of HSP70 were determined of PC12 cells using immunocytochemical approach during heat stress. Results: The expression levels of HSP70 increased in heat stress. In the present study, the proliferations of PC 12 cells also increased. Conclusions: High level expressions of HSP70 is one of protective mechanisms in PC 12 cells.

    【Key words】Heat shock protein 70;Trauma;Heat stress;PC 12 cells

    热休克蛋白 (heat shock proteins,HSP) 70 是一类广泛存在于所有原核和真核细胞内,具有高度保守性的蛋白家族,在正常情况下基础水平表达较低 ......