上海市2003—2010年孕妇弓形虫感染筛查状况分析(1)
【摘要】 目的 了解上海市弓形蟲检测方法和试剂盒使用情况，分析孕妇弓形虫感染状况，为本市弓形虫病的监测防治提供依据。 方法 收集2003-2010年上海市孕妇弓形虫感染筛查的方法和结果资料，分析本市孕妇弓形虫感染筛查状况。 结果 2003-2010年全市30家医疗单位开展孕妇弓形虫感染筛查检测工作，共检测717 391人次，抗体总阳性比例为1.46%，其中IgG和IgM阳性比例分别为2.46%(1.48%-4.96%)和0.83%(0.38%-1.48%)。IgM单项阳性和IgG-IgM双项阳性占抗体阳性的41.74%。春夏季节弓形虫抗体阳性比例高于秋冬季节；不同性质和等级的医疗单位弓形虫抗体阳性检出比例不等。本市应用较多的弓形虫抗体检测方法为酶联免疫吸附试验和化学发光法，且对弓形虫IgG和IgM抗体阳性检出比例不同；进口检测试剂盒筛检弓形虫IgM抗体阳性比例较高，而国产检测试剂盒筛检弓形虫IgG抗体阳性比例较高。结论 上海市2003-2010年孕妇弓形虫抗体水平与本市以往调查结果接近，IgM抗体阳性比例较高，应引起重视；各医疗单位要建立相应的质量控制体系，选用质量稳定可靠的检测试剂盒，避免引起的漏诊和误诊。
关键词：弓形虫；孕妇；筛查
The Screening of Toxoplasma gondii Infection in Shanghai pregncy wormen
ZHU Min， CAI Li*， MA Xing-bao
(Shanghai Municipal Center for Disease Control and Prevention，Shanghai 200336， China)
*Correspondence author
[Abstract] Objective To understand the application status of Toxoplasma Gondii test kit， and analyze the trends of T. gondii infection in Shanghai pregnancy women so as to provide scientific evidences for making out an effective intervention measures to prevent congenital toxoplasmosis. Methods Collect the data of methods and results of screening T. gondii infection in pregnant women in Shanghai from 2003 to 2010， and analysis of T. gondii infection in pregnant women in this city. Results The overall serological positive rate to T. gondii was 1.46%(10454/717391) from 30 hospitals. The positive rates of IgG antibody was 2.46%(1.48%-4.96%) in 277523 person， that of IgM antibody was 0.83%(0.38%-1.48%) in 439868 person. The IgM or both IgG and IgM positive pregnancy cases account for 41.74% (1794/4298) of the total positive cases which appeared in pregnancy women from 2008 to 2010.There were also significantly different in the positive rates among different hospitals so as seasons. ELISA and MEIA were more detected for T. gondii antibody detection， and their detection rates of IgG and IgM were significantly different. Imported kit has higher positive rate of detection T. gondii IgM antibody， but lower positive rate of screening IgG antibody than domestic kit. Conclusion The seroprevalence of T. gondii in pregnancy women from 2003 to 2010 are close to the average level investigated in the past in Shanghai， but more attention should be paid to the higher proportion of IgM antibody positive， and stable and reliable test kits should be selected for detecting T. gondii IgG and IgM antibodies to avoid leak and mistake diagnosis.
[Key words] Toxoplasma gondii； Pregnancy women； Screening, 百拇医药(朱民 蔡黎 马杏宝)
关键词：弓形虫；孕妇；筛查
The Screening of Toxoplasma gondii Infection in Shanghai pregncy wormen
ZHU Min， CAI Li*， MA Xing-bao
(Shanghai Municipal Center for Disease Control and Prevention，Shanghai 200336， China)
*Correspondence author
[Abstract] Objective To understand the application status of Toxoplasma Gondii test kit， and analyze the trends of T. gondii infection in Shanghai pregnancy women so as to provide scientific evidences for making out an effective intervention measures to prevent congenital toxoplasmosis. Methods Collect the data of methods and results of screening T. gondii infection in pregnant women in Shanghai from 2003 to 2010， and analysis of T. gondii infection in pregnant women in this city. Results The overall serological positive rate to T. gondii was 1.46%(10454/717391) from 30 hospitals. The positive rates of IgG antibody was 2.46%(1.48%-4.96%) in 277523 person， that of IgM antibody was 0.83%(0.38%-1.48%) in 439868 person. The IgM or both IgG and IgM positive pregnancy cases account for 41.74% (1794/4298) of the total positive cases which appeared in pregnancy women from 2008 to 2010.There were also significantly different in the positive rates among different hospitals so as seasons. ELISA and MEIA were more detected for T. gondii antibody detection， and their detection rates of IgG and IgM were significantly different. Imported kit has higher positive rate of detection T. gondii IgM antibody， but lower positive rate of screening IgG antibody than domestic kit. Conclusion The seroprevalence of T. gondii in pregnancy women from 2003 to 2010 are close to the average level investigated in the past in Shanghai， but more attention should be paid to the higher proportion of IgM antibody positive， and stable and reliable test kits should be selected for detecting T. gondii IgG and IgM antibodies to avoid leak and mistake diagnosis.
[Key words] Toxoplasma gondii； Pregnancy women； Screening, 百拇医药(朱民 蔡黎 马杏宝)