应用G-CSF动员骨髓干细胞治疗重型颅脑损伤的研究(1)
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2010年8月1日
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【摘要】探究重组人粒细胞集落刺激因子(rh-G-CSF)治疗重型颅脑损伤的有效性及临床应用安全性。方法将60例重型颅脑损伤患者,随机分成实验组30人,对照组30人。实验组连续3天给予G-CSF 300μg/天。应用流式细胞仪监测并比较治疗前后外周血中CD34+和CD133+细胞计数及磁共振波普分析(MRS)评价治疗前后受损部位的N-乙酰氨基酸(NAA)含量,常规评价其功能恢复情况。监测外周血白细胞计数评价其安全性。结果实验组治疗后CD34+、CD133+细胞计数和受损部位的NAA含量较治疗前明显增加(p<0.05),白细胞计数同步增加(P<0.05)。治疗1个月时实验组GCS评分较对照组略有改善,但差异无显著性(t=0.032,P>0.05),随访3个月、6个月时GCS评分较对照组均明显升高(t=1.418,1.592,P<0.05)且随访6个月时实验组的疗效明显优于对照组(Z =3.576,P<0.01),实验组未出现严重不良反应。结论用G-CSF动员骨髓干细胞迁移至脑损伤区域并分化为神经细胞,明显改善神经功能。
【关键词】粒细胞集落刺激因子不达意 重型颅脑损伤干细胞神经功能
中国图分类号:R651.5文献标识码:A 文章编号:1005-0515(2010)08-001-03
Granulocyte colony stimulating factor-mobilized autologous bone marrow nuclear
cell transplantation in treatment of patients with severe traumatic brain injury
XUE Hongli,ZHOU Jiang,YU Chunyong,LIU Minpei,SHAO Shuangwei,XU Feng,WANG Zhijun,LIN Di
Department of Neurosurgery, The General Hospital of Shenyang Military Region, Shen yang 110016, Liaoning Province, PR China.
【ABSTRACT】The aim of this study was to assess the safety and efficacy of treatment for patients with severe traumatic brain injury (sTBI) with transplantation of granulocyte colony stimulating factor(G-CSF) -mobilized autologous bone marrow stem cells (BMSCs) MethodsSixty patients with sTBI were randomly divided into the control group (group A),the group treated with transplantation of G-CSF -mobilized BMSCs (group B,G-CSF ,300μg/day ×3 days).ResultsCount of CD 34+ and CD 133+ cells in peripheral blood. was observed by flow cytometry. N- acetylaspartate (NAA) was measured by magnetic resonance spectroscopy (MRS) in brain tissue .The function of the nervous system in patients was measured by using Glasgow Coma Scale (GCS) scores . Count of white blood cells was performed to assess the safety of treatment.Count of CD 34+ and CD 133+ cells in peripheral blood. and NAA expression significantly increased after treatment withtransplantation of G-CSF-mobilized autologous BMSCs (p<0 ......
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