摘要：目的 建立一测多评法(QAMS)测定胆黄连中3种生物碱成分含量，并与外标法测定结果进行比较，探讨QAMS的可行性。方法 采用高效液相色谱法，以盐酸小檗碱为内参物，建立盐酸小檗碱与盐酸巴马汀、盐酸药根碱间的相对校正因子，用所得相对校正因子进行含量计算(计算值)，实现一测多评。同时采用外标法测定3个成分的含量(实测值)，比较计算值与实测值的差异。结果 10批胆黄连中3种生物碱成分含量的计算值与实测值无显著差异。结论 QAMS可用于胆黄连中生物碱类成分的含量测定。

    关键词：一测多评法；外标法；胆黄连；生物碱；相对校正因子

    DOI：10.3969/j.issn.1005-5304.2016.11.019

    中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2016)11-0078-03

    Abstract： Objective To establish QAMS method to determine the contents of three alkaloids in bile processed Coptidis Rhizoma； To compare the results of QAMS with those from external standard method； To prove the feasibility of QAMS. Methods An HPLC method was developed. Berberine hydrochloride was selected as the internal reference substance. 2 relative correction factors (RCF) of berberine hydrochloride to palmatine hydrochloride and to jatrorrhizine hydrochloride were established. Obtained RCFs were used to conduct content calculation (calculated value) to complete QAMS method. At the same time ......