逍遥抗癌解郁方对乳腺癌并发抑郁症模型小鼠抑郁样指征的影响(1)
摘要：目的 觀察逍遥抗癌解郁方对乳腺癌并发抑郁症模型小鼠的影响，探讨其作用机制。方法 以BALB/c雌性小鼠为研究对象，采用腋下注射4T1炎性乳腺癌细胞联合腹腔注射皮质酮(CORT)建立乳腺癌并发抑郁症模型小鼠。根据糖水消耗量随机分为空白组、模型组、紫杉醇组、逍遥抗癌解郁方组、化疗+氟西汀组、化疗+逍遥抗癌解郁方组，分别给予相应药物21 d后，采用糖水偏好实验、Open-field实验和新奇摄食实验检测小鼠行为学变化；ELISA检测小鼠血浆促肾上腺激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、CORT，血清5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)，肿瘤标志物糖类抗原CA153、CA125和癌胚抗原(CEA)的含量。结果 与空白组比较，模型组小鼠糖水偏好度、水平与垂直运动明显降低(P<0.01，P<0.05)，摄食潜伏期明显增加(P<0.01，P<0.05)，肿瘤标志物CA153、CA125和CEA含量及下丘脑-垂体-肾上腺轴相关指标显著升高(P<0.01)；单胺神经递质NE、DA和5-HT明显下降(P<0.01)；与模型组比较，化疗+逍遥抗癌解郁方组小鼠糖水消耗量显著升高，自主行为活动增加，行为绝望状态改善(P<0.01，P<0.05)；肿瘤标物及HPA轴相关指标含量明显降低(P<0.01，P<0.05)；5-HT、DA和NE含量明显升高(P<0.01，P<0.05)。结论 逍遥抗癌解郁方联合紫杉醇可有效缓解乳腺癌并发抑郁症的抑郁样指征。
关键词：逍遥抗癌解郁方；乳腺癌并发抑郁症；单胺神经递质；下丘脑-垂体-肾上腺轴；小鼠
DOI：10.3969/j.issn.1005-5304.2018.11.011
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2018)11-0046-06
Abstract： Objective To investigate the effects of Xiaoyao Kang'ai Jieyu Formula on mice with breast cancer related depression； To explore its mechanism. Methods BALB/c female mice were set as study objects. Subaxillary injection of inflammatory 4T1 cells and intraperitoneal injection of corticosterone were used to establish a model of breast cancer related depression. According to the consumption of sugar water， mice were randomly divided into blank group， model group， paclitaxel group， Xiaoyao Kang'ai Jieyu Formula group， chemotherapy + fluoxetine group， and chemotherapy + Xiaoyao Kang'ai Jieyu Formula group. After 21 days， sucrose preference test， open-field test， and novelty feeding test were performed to detect changes in the behavior of mice in each group. ELISA were used to detected the contents of corticotrophin releasing hormone (CRH)， adrenocorticotropic hormone (ACTH)， and corticosterone (CORT) in plasma， and the contents of 5-hydroxytryptamine (5-HT)， dopamine (DA)， norepinephrine(NE)， tumor markers carbohydrate antigen CA153， CA125 and carcinoembryonic antigen (CEA) in serum. Results Compared with the blank group， the consumption of sugar， horizontal and vertical movements of mice in the model group significantly decreased (P<0.01， P<0.05)； the latency of ingestion significantly increased (P<0.01， P<0.05)； the levels of tumor markers CA153， CA125， CEA and some indexes of HPA axis significantly increased (P<0.01)； the expressions of monoamine neurotransmitter NE， DA and 5-HT significantly decreased (P<0.01). Compared with the model group， sugar consumption and autonomic activity significantly increased in the chemotherapy + Xiaoyao Kang'ai Jieyu Formula group， and behavioral despair condition was improved (P<0.01， P<0.05)； the content of tumor markers and some indexes related to HPA axis significantly decreased (P<0.01， P<0.05)； the contents of 5-HT， DA and NE significantly increased (P<0.01， P<0.05). Conclusion The combination of Xiaoyao Kang'ai Jieyu Formula and paclitaxel can effectively relieve the depressive symptoms of breast cancer related depression., 百拇医药(孟盼 黄会珍 杨琴 赵洪庆 杨蕙 韩远山 凌佳 王宇红)
关键词：逍遥抗癌解郁方；乳腺癌并发抑郁症；单胺神经递质；下丘脑-垂体-肾上腺轴；小鼠
DOI：10.3969/j.issn.1005-5304.2018.11.011
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2018)11-0046-06
Abstract： Objective To investigate the effects of Xiaoyao Kang'ai Jieyu Formula on mice with breast cancer related depression； To explore its mechanism. Methods BALB/c female mice were set as study objects. Subaxillary injection of inflammatory 4T1 cells and intraperitoneal injection of corticosterone were used to establish a model of breast cancer related depression. According to the consumption of sugar water， mice were randomly divided into blank group， model group， paclitaxel group， Xiaoyao Kang'ai Jieyu Formula group， chemotherapy + fluoxetine group， and chemotherapy + Xiaoyao Kang'ai Jieyu Formula group. After 21 days， sucrose preference test， open-field test， and novelty feeding test were performed to detect changes in the behavior of mice in each group. ELISA were used to detected the contents of corticotrophin releasing hormone (CRH)， adrenocorticotropic hormone (ACTH)， and corticosterone (CORT) in plasma， and the contents of 5-hydroxytryptamine (5-HT)， dopamine (DA)， norepinephrine(NE)， tumor markers carbohydrate antigen CA153， CA125 and carcinoembryonic antigen (CEA) in serum. Results Compared with the blank group， the consumption of sugar， horizontal and vertical movements of mice in the model group significantly decreased (P<0.01， P<0.05)； the latency of ingestion significantly increased (P<0.01， P<0.05)； the levels of tumor markers CA153， CA125， CEA and some indexes of HPA axis significantly increased (P<0.01)； the expressions of monoamine neurotransmitter NE， DA and 5-HT significantly decreased (P<0.01). Compared with the model group， sugar consumption and autonomic activity significantly increased in the chemotherapy + Xiaoyao Kang'ai Jieyu Formula group， and behavioral despair condition was improved (P<0.01， P<0.05)； the content of tumor markers and some indexes related to HPA axis significantly decreased (P<0.01， P<0.05)； the contents of 5-HT， DA and NE significantly increased (P<0.01， P<0.05). Conclusion The combination of Xiaoyao Kang'ai Jieyu Formula and paclitaxel can effectively relieve the depressive symptoms of breast cancer related depression., 百拇医药(孟盼 黄会珍 杨琴 赵洪庆 杨蕙 韩远山 凌佳 王宇红)