维吾尔族患者腰椎间盘组织中IL—6的含量及临床相关性(1)
摘要：目的 检测促炎因子白介素-6(IL-6)和抗炎因子白介素-10(IL-10)在人腰椎间盘组织中的表达含量，并探讨其与临床相关性。方法 收集30例单节段椎间盘突出症维吾尔族患者及10例非腰椎源性死亡的新鲜尸体的腰椎间盘组织，术前由一名受培训的医师进行详细病史询问，明确病程长短，对术前临床症状均给予JOA(下腰痛JOA评分)评定，术后应用酶联免疫吸附测定法(ELISA)测定椎间盘组织中IL-6和IL-10的含量。结果 維吾尔族突出腰椎间盘组织中IL-6含量较对照组高(P<0.01)，有统计学意义。脱出型腰椎间盘组织中病例组IL-6含量高于突出性(P<0.01)，有统计学意义。病例组IL-6和患者病程长短呈负相关，IL-6和患者JOA评分呈负相关。结论 突出的腰椎间盘组织有分泌炎症因子的功能术前病程长短与患者手术前下腰痛引起的腰椎功能情况变化有相关性，细胞因子是相互依赖、相互影响的，且与临床密切相关。
关键词：椎间盘突出；细胞因子；相关性
The Content and Clinical Correlation of IL-6 in the Lumbar Intervertebral Disc Tissue of Uygur Patients
, 百拇医药
Xuhereti·Wutikuerhabaxi·Kaken，YUAN Hong
(Department of Joint Surgery，The Xinjiang Uygur Autonomous Region people's Hospital，Urumqi 830001，Xinjiang，China)
Abstract：Objective The expression contents of pro-inflammatory cytokine IL-6 and in the intervertebral disc and its significance and correlation with clinical findings in Uygur people.Methods Specimens of herniated lumbar disc from 30 Uygur cases of disc herniation in single level and other 10 Specimens from corpse not dead from spinal disease.The clinical symptoms in all cases were evaluated by JOA index preparation，and make sure the patient's history，that all done by one doctor.The contents of IL-6 in the intervertebral disc were detected by enzyme-linked immunosorbent assay.Results The expression levels of IL-6 in Uygur people was significantly higher in the patients than in the controls.Patient's history is having negative correlation with IL-6 contents in the intervertebral disc.Contents of IL-6 in the extrusion intervertebral disc were higher in herniation.Patient's history is have correlation with Patient's JOA index，Patient's JOA index have negative correlation with IL-6.Conclusion The herniated lumbar disc have abilities of excrete pro-inflammatory of cytokines.Patient's history is having correlation with Patient's JOA index.Cytokines is not alone，they react to each other，correlation with clinical findings.
, 百拇医药
Key words：Intervertebral disc herniation；Cytokines；Correlation
腰椎间盘突出症为骨科的常见病和多发病。近年来，本病的发病率有逐渐上升的趋势[1]。腰椎间盘退行性变是导致腰椎间盘突出症的基本病因。椎间盘退变后，髓核内的蛋白多糖逐渐减少，含水量不断下降，关节面的应力结构受损并影响脊柱其他结构，最终引起脊柱退变。目前认为其不仅仅与椎间盘的生物力学改变等机械性因素有关，生物细胞因子也可成为椎间盘退变的重要原因[2-3]。
1资料与方法
1.1一般资料 病例组入选条件①有手术指征：经临床检查、影像学检查及手术确诊为腰椎间盘突出症患者；②排除脊柱畸形滑脱、炎症、肿瘤、结核及免疫系统疾病等影响因素；③无其他可能影响神经功能的疾病(如：糖尿病)；④单节段椎间盘突出的住院患者。
我院2013年1月～2014年1月的住院30例维吾尔族患者，其中男15例，女15例；年龄24～65岁，平均42岁；L3/4椎间盘3例，L4/5椎间盘14例，L5/S1椎间盘13例。30例椎间盘突出症患者经手术证实突出型21例，脱出型9例，无膨出型。所有患者均有较典型的腰腿痛症状，无急慢性感染、糖尿病等其余病史，术前由一名受培训的医师进行详细病史询问，明确病程长短，对术前临床症状均给予日本骨科学会腰椎功能评分标准评定(JOA评分)。对照组取10例非腰椎源性疾病死亡的新鲜尸体的腰椎间盘组织。其中男4例，女6例；年龄28～65岁，平均44.6岁，标本为4例L4/5，6例L5/S1，术中见椎间盘完整无突出表现。, http://www.100md.com(旭合热提·吾提库尔哈巴西·卡肯 袁宏)
关键词：椎间盘突出；细胞因子；相关性
The Content and Clinical Correlation of IL-6 in the Lumbar Intervertebral Disc Tissue of Uygur Patients
, 百拇医药
Xuhereti·Wutikuerhabaxi·Kaken，YUAN Hong
(Department of Joint Surgery，The Xinjiang Uygur Autonomous Region people's Hospital，Urumqi 830001，Xinjiang，China)
Abstract：Objective The expression contents of pro-inflammatory cytokine IL-6 and in the intervertebral disc and its significance and correlation with clinical findings in Uygur people.Methods Specimens of herniated lumbar disc from 30 Uygur cases of disc herniation in single level and other 10 Specimens from corpse not dead from spinal disease.The clinical symptoms in all cases were evaluated by JOA index preparation，and make sure the patient's history，that all done by one doctor.The contents of IL-6 in the intervertebral disc were detected by enzyme-linked immunosorbent assay.Results The expression levels of IL-6 in Uygur people was significantly higher in the patients than in the controls.Patient's history is having negative correlation with IL-6 contents in the intervertebral disc.Contents of IL-6 in the extrusion intervertebral disc were higher in herniation.Patient's history is have correlation with Patient's JOA index，Patient's JOA index have negative correlation with IL-6.Conclusion The herniated lumbar disc have abilities of excrete pro-inflammatory of cytokines.Patient's history is having correlation with Patient's JOA index.Cytokines is not alone，they react to each other，correlation with clinical findings.
, 百拇医药
Key words：Intervertebral disc herniation；Cytokines；Correlation
腰椎间盘突出症为骨科的常见病和多发病。近年来，本病的发病率有逐渐上升的趋势[1]。腰椎间盘退行性变是导致腰椎间盘突出症的基本病因。椎间盘退变后，髓核内的蛋白多糖逐渐减少，含水量不断下降，关节面的应力结构受损并影响脊柱其他结构，最终引起脊柱退变。目前认为其不仅仅与椎间盘的生物力学改变等机械性因素有关，生物细胞因子也可成为椎间盘退变的重要原因[2-3]。
1资料与方法
1.1一般资料 病例组入选条件①有手术指征：经临床检查、影像学检查及手术确诊为腰椎间盘突出症患者；②排除脊柱畸形滑脱、炎症、肿瘤、结核及免疫系统疾病等影响因素；③无其他可能影响神经功能的疾病(如：糖尿病)；④单节段椎间盘突出的住院患者。
我院2013年1月～2014年1月的住院30例维吾尔族患者，其中男15例，女15例；年龄24～65岁，平均42岁；L3/4椎间盘3例，L4/5椎间盘14例，L5/S1椎间盘13例。30例椎间盘突出症患者经手术证实突出型21例，脱出型9例，无膨出型。所有患者均有较典型的腰腿痛症状，无急慢性感染、糖尿病等其余病史，术前由一名受培训的医师进行详细病史询问，明确病程长短，对术前临床症状均给予日本骨科学会腰椎功能评分标准评定(JOA评分)。对照组取10例非腰椎源性疾病死亡的新鲜尸体的腰椎间盘组织。其中男4例，女6例；年龄28～65岁，平均44.6岁，标本为4例L4/5，6例L5/S1，术中见椎间盘完整无突出表现。, http://www.100md.com(旭合热提·吾提库尔哈巴西·卡肯 袁宏)