母婴分离对新生期大鼠成年后海马组织激肽释放酶8的影响(1)
摘要：目的 通过检测和观察母婴分离致大鼠海马区KLK8的分泌表达水平变化，探讨新生期母婴分离导致大鼠成年后行为异常的可能机制。方法 取新生Wistar大鼠24只，即刻分为对照组和分离组，12只为一组，其中将分离组的Wistar鼠在出生后的次日与母亲分离，对照组大鼠与母鼠同笼。出生6周后，处死Wistar大鼠，取海马组织，HE染色后切片，取出大鼠海马，检测其海马组织中klk8的水平。应用Image-Pro Plus (IPP) 6.0软件系统对免疫组化多个图像逐一做半定量分析。结果 ：经过母婴分离的Wistar大鼠，在其成年后，kLK8的表达与对照组相比明显降低 (P<0.01)，提示这种差异有统计学意义。 结论 大鼠在新生期进行母婴分离，可致大鼠海马KLK8的表达出现下降，这对神经信号可产生影响，使其突触的可塑性出现变化，随后对行为产生影响。
关键词：母婴分离；海马；激肽释放酶8
Effects of Maternal and Neonatal Isolation on KLK8 of Hippocampus in Neonatal Rats
, 百拇医药
ZHANG Lu1，DONG Wen-bin2
(1.Neonatal Department，Maternal and Child Health Care Hospital of Luzhou，Luzhou 646000，Sichuan，China；2.Neonatal Department，The Affiliated Hospital of Southwest Medical University，Luzhou 646000，Sichuan，China)
Abstract：Objective To detect the expression level of KLK8 in rats' hippocampal tissue after maternal separation， and explore the potential mechanism of rats' abnormal behavior resulted from maternal separation in neonatal period. Methods 24 Wistar neonatal rats were divided into maternal separation group and control group. Nothing was done to the rats in the control group. After 6 weeks， rats were killed.After the hippocampus tissue were determined by use of HE staining ， The expression of KLK8 in rat's hippocampal tissue were detected by immunohistochemistry. Image-Pro Plus software which were used to semiquantitative assessment the immuneohistochemical images. Results Immunohistochemistry showed that KLK8 expression level in hippocampal tissue were significantly decreased in NMS group compared with the control group (P<0.01). Conclusion The expression of KLK8 were decreased in hippocampatissue after maternal separation， may to a certain extent affected the neural signal transduction and synaptic plasticity，which are possibly related to the rats' abnormal behavior.
, 百拇医药
Key words：Maternal separation； Hippocampus； Tissue kallikrein 8； Abnormal behavior
研究證实，早期应激事件会导致成人后发生心理疾病，例如，在童年的家庭暴力，在成年后很容易出现心境障碍或创伤后应激障碍(post-traumatic disorder，PTSD)[1-3]。
当代研究表明，长时间的母婴分离(Neonate Mother Separation，NMS)可能造成成年后学习记忆力减退，认知障碍，还会引起焦虑样的行为等，过早的母婴分离会导致人体应激系统功能障碍和异常行为，这种负面影响在青少年时期甚至成年后依然存在[4]。由母婴分离的大鼠动物模型可知，如果在大鼠新生期给以母婴分离后，大鼠成年后自发活动减少，出现认知力差，精神焦虑抑郁，对内脏疼痛更加敏感，神经元重塑也会受损害[5-6]。
组织激肽释放酶(tissue kallikrein，KLK)是一种分泌型丝氨酸蛋白酶，参与学习和记忆的过程。人类的KLK8分布在许多正常组织，包括皮肤，食道，睾丸，扁桃体，肾，乳腺表达，和唾液腺[7]，越来越多的证据表明在发展神经蛋白酶，成熟，老化和认知功能的方面。
研究表明，就发育水平而言，出生后2周岁儿童的发育水平与28 d的大鼠发育程度相当[8]，本研究在前期建立大鼠母婴分离模型基础上，以KLK8为出发点，探讨母婴分离的大鼠海马KLK8诱导表达水平的变化，以研究新生期母婴分离可能会导致大鼠成年后异常行为的机制。, http://www.100md.com(张璐 董文斌)
关键词：母婴分离；海马；激肽释放酶8
Effects of Maternal and Neonatal Isolation on KLK8 of Hippocampus in Neonatal Rats
, 百拇医药
ZHANG Lu1，DONG Wen-bin2
(1.Neonatal Department，Maternal and Child Health Care Hospital of Luzhou，Luzhou 646000，Sichuan，China；2.Neonatal Department，The Affiliated Hospital of Southwest Medical University，Luzhou 646000，Sichuan，China)
Abstract：Objective To detect the expression level of KLK8 in rats' hippocampal tissue after maternal separation， and explore the potential mechanism of rats' abnormal behavior resulted from maternal separation in neonatal period. Methods 24 Wistar neonatal rats were divided into maternal separation group and control group. Nothing was done to the rats in the control group. After 6 weeks， rats were killed.After the hippocampus tissue were determined by use of HE staining ， The expression of KLK8 in rat's hippocampal tissue were detected by immunohistochemistry. Image-Pro Plus software which were used to semiquantitative assessment the immuneohistochemical images. Results Immunohistochemistry showed that KLK8 expression level in hippocampal tissue were significantly decreased in NMS group compared with the control group (P<0.01). Conclusion The expression of KLK8 were decreased in hippocampatissue after maternal separation， may to a certain extent affected the neural signal transduction and synaptic plasticity，which are possibly related to the rats' abnormal behavior.
, 百拇医药
Key words：Maternal separation； Hippocampus； Tissue kallikrein 8； Abnormal behavior
研究證实，早期应激事件会导致成人后发生心理疾病，例如，在童年的家庭暴力，在成年后很容易出现心境障碍或创伤后应激障碍(post-traumatic disorder，PTSD)[1-3]。
当代研究表明，长时间的母婴分离(Neonate Mother Separation，NMS)可能造成成年后学习记忆力减退，认知障碍，还会引起焦虑样的行为等，过早的母婴分离会导致人体应激系统功能障碍和异常行为，这种负面影响在青少年时期甚至成年后依然存在[4]。由母婴分离的大鼠动物模型可知，如果在大鼠新生期给以母婴分离后，大鼠成年后自发活动减少，出现认知力差，精神焦虑抑郁，对内脏疼痛更加敏感，神经元重塑也会受损害[5-6]。
组织激肽释放酶(tissue kallikrein，KLK)是一种分泌型丝氨酸蛋白酶，参与学习和记忆的过程。人类的KLK8分布在许多正常组织，包括皮肤，食道，睾丸，扁桃体，肾，乳腺表达，和唾液腺[7]，越来越多的证据表明在发展神经蛋白酶，成熟，老化和认知功能的方面。
研究表明，就发育水平而言，出生后2周岁儿童的发育水平与28 d的大鼠发育程度相当[8]，本研究在前期建立大鼠母婴分离模型基础上，以KLK8为出发点，探讨母婴分离的大鼠海马KLK8诱导表达水平的变化，以研究新生期母婴分离可能会导致大鼠成年后异常行为的机制。, http://www.100md.com(张璐 董文斌)