芪参二莲汤对肝纤维化大鼠基质金属蛋白酶—1及抑制因子的影响(1)
摘 要：目的 研究不同剂量芪参二莲汤对肝纤维化大鼠基质金属蛋白酶-1及抑制因子变化的影响。方法 于2017年6月～8月选择90只SD雄性大鼠作为研究对象，按照体质量随机分为6组，每组15只，其中1组为正常组，其余5组均为肝纤维化模型，将其分为模型组、秋水仙碱组以及芪参二莲汤低剂量组(5 g/kg)、中剂量组(10 g/kg)和高剂量组(15 g/kg)；各给药组分别给予相应的药物灌胃，正常组、模型组则给予生理盐水灌胃；对比各组大鼠MMP-1和TIMP-1水平以及肝纤维化指标水平。结果 正常组与模型组比较，后者的MMP-1、MMP-1/TIMP-1水平明显降低，而TLISA-1水平则明显升高，差异有统计学意义(P<0.05)；模型组与各给药组比较，各给药组的MMP-1、MMP-1/TIMP-1水平较模型组升高，而TIMP-1水平则较模型组降低，差异有统计学意义(P<0.05)，且给药组中，以芪参二莲汤高剂量组的变化水平最为显著，差异有统计学意义(P<0.05)。结论 15 g/kg高剂量的芪参二莲汤用于治疗肝纤维化大鼠，能够有效促进MMP-1表达、减少TIMP-1表达，进而起到逆转肝纤维化的作用。
, 百拇医药
關键词：芪参二莲汤；肝纤维化；基质金属蛋白酶-1；抑制因子
中图分类号：R285.5 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.15.045
文章编号：1006-1959(2018)15-0140-03
Effects of Qishenerlian Decoction on Matrix Metalloproteinase-1 and Its Inhibitory Factors in Rats with Hepatic Fibrosis
AI Ying-chun，CHI Nan-nan，DU Jing-feng，ZHANG Xue-mei
(Department of Digestion，Subject Two，the First Affiliated Hospital of Jiamusi University，Jiamusi 154002，Heilongjiang，China)
, http://www.100md.com
Abstract：Objective To study the effects of different doses of Qishenerlian Decoction on matrix metalloproteinase-1 and inhibitor of hepatic fibrosis in rats.Methods In June to August 2017， 90 male SD rats were selected as the research subjects.According to the body mass，they were randomly divided into 6 groups，with 15 rats in each group，of which 1 was the normal group and the other 5 groups were liver fibrosis models.They were divided into model group，colchicine group and low dose group of Qishenerlian Decoction (5g/kg)，medium dose group(10g/kg)and high dose group(15g/kg).Each drug-administered group was given the corresponding drugs，and the normal group and the model group were given normal saline.The levels of MMP-1 and TIMP-1 and liver fibrosis were compared between the groups.Results Compared with the normal group，the level of MMP-1 and MMP-1/TIMP-1 in the model group was significantly decreased，while the level of TLISA-1 was significantly higher，the difference was statistically significant(P<0.05)；Compared with the drug-administered group，the MMP-1 and MMP-1/TIMP-1 levels in the model group were higher than those in the model group，while the TIMP-1 level was lower than that in the model group，the difference was statistically significant(P<0.05)，and in the drug-administered group，the change level of the high-dose group of Qishenerlian Decoction was the most significant，and the difference was statistically significant(P<0.05).Conclusion The high dose Qishenerlian Decoction for 15g/kg can effectively promote the expression of MMP-1，reduce the expression of TIMP-1 and reverse the hepatic fibrosis in rats., http://www.100md.com(艾迎春 迟男男 杜景峰 张雪梅)
, 百拇医药
關键词：芪参二莲汤；肝纤维化；基质金属蛋白酶-1；抑制因子
中图分类号：R285.5 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.15.045
文章编号：1006-1959(2018)15-0140-03
Effects of Qishenerlian Decoction on Matrix Metalloproteinase-1 and Its Inhibitory Factors in Rats with Hepatic Fibrosis
AI Ying-chun，CHI Nan-nan，DU Jing-feng，ZHANG Xue-mei
(Department of Digestion，Subject Two，the First Affiliated Hospital of Jiamusi University，Jiamusi 154002，Heilongjiang，China)
, http://www.100md.com
Abstract：Objective To study the effects of different doses of Qishenerlian Decoction on matrix metalloproteinase-1 and inhibitor of hepatic fibrosis in rats.Methods In June to August 2017， 90 male SD rats were selected as the research subjects.According to the body mass，they were randomly divided into 6 groups，with 15 rats in each group，of which 1 was the normal group and the other 5 groups were liver fibrosis models.They were divided into model group，colchicine group and low dose group of Qishenerlian Decoction (5g/kg)，medium dose group(10g/kg)and high dose group(15g/kg).Each drug-administered group was given the corresponding drugs，and the normal group and the model group were given normal saline.The levels of MMP-1 and TIMP-1 and liver fibrosis were compared between the groups.Results Compared with the normal group，the level of MMP-1 and MMP-1/TIMP-1 in the model group was significantly decreased，while the level of TLISA-1 was significantly higher，the difference was statistically significant(P<0.05)；Compared with the drug-administered group，the MMP-1 and MMP-1/TIMP-1 levels in the model group were higher than those in the model group，while the TIMP-1 level was lower than that in the model group，the difference was statistically significant(P<0.05)，and in the drug-administered group，the change level of the high-dose group of Qishenerlian Decoction was the most significant，and the difference was statistically significant(P<0.05).Conclusion The high dose Qishenerlian Decoction for 15g/kg can effectively promote the expression of MMP-1，reduce the expression of TIMP-1 and reverse the hepatic fibrosis in rats., http://www.100md.com(艾迎春 迟男男 杜景峰 张雪梅)