人脂肪干细胞与VEGF—PLGA纳米微球缓释系统构建工程化脂肪组织的相关研究(1)
摘 要：目的 探讨缓释血管内皮生长因子复合支架与脂肪干细胞构建工程化脂肪的可行性。方法 制备VEGF-PLGA纳米微球缓释支架，检测支架释放 VEGF 浓度，体外提取培养 ADSCs，检测复合支架对 ASC 生长增殖的影响。将复合支架和ADSCs植入裸小鼠背部，8 周后切取植入物称重，组织切片HE染色，评估效果。结果 VEGF-PLGA纳米微球缓释复合支架能连续 12 d释放较高浓度的VEGF，对 ADSCs的生长增殖无影响。动物实验结果显示复合支架与ADSCs共移植能显著提高脂肪组织的形成，增加血管的生成(P<0.01)，减少组织坏死。结论 VEGF-PLGA纳米微球缓释系统具有良好的生物安全性，其与ADSCs共移植构建工程化脂肪组织具有一定的可行性。
关键词：血管内皮生长因子；缓释支架；脂肪干细胞；工程化脂肪
中图分类号：R622+.9 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.22.018
, 百拇医药
文章編号：1006-1959(2018)22-0064-03
Study on the Relationship between Human Adipose Stem Cells and VEGF-PLGA Nanospheres Slow-release System for Construction of Engineered Adipose Tissue
WANG Yong-heng1，LI Lin-rui1 ，LEI Yu2，HUO Bin-liang1，CAO Wei1
( Department of Surgical Oncology1，Department of Oncology2，Shaanxi Provincial People's Hospital，Xi'an 710068，Shaanxi，China)
, 百拇医药
Abstract：Objective To investigate the feasibility of construction of engineered adipose tissue with sustained-release vascular endothelial growth factor(VEGF) composite stent and adipose stem cells.Methods VEGF-PLGA nanospheres sustained-release scaffolds were prepared and the concentration of VEGF was measured. ADSCs， was extracted in vitro to detect the effect of composite scaffolds on the growth and proliferation of ASC.The composite scaffold and ADSCs were implanted into the back of nude mice. After 8 weeks， the implants were weighed， and the tissue sections were stained with HE to evaluate the effect.Results VEGF-PLGA nanospheres sustained-release composite scaffolds could release higher concentration of VEGF， for 12 d without any effect on the growth and proliferation of ADSCs.The results of animal experiments showed that co-transplantation of composite scaffold and ADSCs could significantly increase the formation of adipose tissue， increase angiogenesis (P<0. 01)， and reduce tissue necrosis.Conclusion VEGF-PLGA nanospheres sustained-release system has good biological safety and it is feasible to co-transplant with ADSCs to construct engineered adipose tissue.
, 百拇医药
Key words：Vascular endothelial growth factor；Sustained-release scaffold；Adipose-derived stem cells；Engineered adipose tissue
脂肪干细胞 (adipose-derived stem cells，ADSCs)是来源于脂肪组织的一种间充质干细胞，具有多向分化潜能，其应用于乳房重建的优势是组织来源充足、取材简便，同时其无免疫排异的风险，能用于自体移植、同种异体移植，甚至异种移植[1]。临床上常见有因乳房发育不良、肿瘤切除、外伤等原因引起的小乳症、乳房的缺失等，可能引起患者严重的身心障碍。现有的乳房填充材料存在生物相容性差、结构稳定性差等缺点。寻找合适的乳房重建材料是目前乳腺外科研究的重点。自体脂肪组织是目前进行乳房填充和重建的最佳材料，但其来源受到很大限制，并且效果不稳定。自体脂肪颗粒移植存活率低，且易发生坏死、液化、纤维化、钙化等，难以大量获取，故难以广泛应用。而随着材料学的发展，各种缓释支架的应用在为诱导干细胞分化、促进血管生成和增加血供并提高植入物的存活率提供了新的思路[2，3]。本研究首先提取培养 ADSCs，再制备能血管内皮生长因子(vascular endothelial growth factor，VEGF) 缓释聚乳酸-羟基乙酸共聚物纳米微球复合支架(polylactic-co-glycolic acid，PLGA)，将ADSCs与复合支架植入裸小鼠的背部，通过测量植入物的重量、组织切片 HE 染色观察，探讨ADSCs和复合支架移植进行组织工程化脂肪构建的可行性。, http://www.100md.com(王永恒 李林瑞 雷雨)
关键词：血管内皮生长因子；缓释支架；脂肪干细胞；工程化脂肪
中图分类号：R622+.9 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.22.018
, 百拇医药
文章編号：1006-1959(2018)22-0064-03
Study on the Relationship between Human Adipose Stem Cells and VEGF-PLGA Nanospheres Slow-release System for Construction of Engineered Adipose Tissue
WANG Yong-heng1，LI Lin-rui1 ，LEI Yu2，HUO Bin-liang1，CAO Wei1
( Department of Surgical Oncology1，Department of Oncology2，Shaanxi Provincial People's Hospital，Xi'an 710068，Shaanxi，China)
, 百拇医药
Abstract：Objective To investigate the feasibility of construction of engineered adipose tissue with sustained-release vascular endothelial growth factor(VEGF) composite stent and adipose stem cells.Methods VEGF-PLGA nanospheres sustained-release scaffolds were prepared and the concentration of VEGF was measured. ADSCs， was extracted in vitro to detect the effect of composite scaffolds on the growth and proliferation of ASC.The composite scaffold and ADSCs were implanted into the back of nude mice. After 8 weeks， the implants were weighed， and the tissue sections were stained with HE to evaluate the effect.Results VEGF-PLGA nanospheres sustained-release composite scaffolds could release higher concentration of VEGF， for 12 d without any effect on the growth and proliferation of ADSCs.The results of animal experiments showed that co-transplantation of composite scaffold and ADSCs could significantly increase the formation of adipose tissue， increase angiogenesis (P<0. 01)， and reduce tissue necrosis.Conclusion VEGF-PLGA nanospheres sustained-release system has good biological safety and it is feasible to co-transplant with ADSCs to construct engineered adipose tissue.
, 百拇医药
Key words：Vascular endothelial growth factor；Sustained-release scaffold；Adipose-derived stem cells；Engineered adipose tissue
脂肪干细胞 (adipose-derived stem cells，ADSCs)是来源于脂肪组织的一种间充质干细胞，具有多向分化潜能，其应用于乳房重建的优势是组织来源充足、取材简便，同时其无免疫排异的风险，能用于自体移植、同种异体移植，甚至异种移植[1]。临床上常见有因乳房发育不良、肿瘤切除、外伤等原因引起的小乳症、乳房的缺失等，可能引起患者严重的身心障碍。现有的乳房填充材料存在生物相容性差、结构稳定性差等缺点。寻找合适的乳房重建材料是目前乳腺外科研究的重点。自体脂肪组织是目前进行乳房填充和重建的最佳材料，但其来源受到很大限制，并且效果不稳定。自体脂肪颗粒移植存活率低，且易发生坏死、液化、纤维化、钙化等，难以大量获取，故难以广泛应用。而随着材料学的发展，各种缓释支架的应用在为诱导干细胞分化、促进血管生成和增加血供并提高植入物的存活率提供了新的思路[2，3]。本研究首先提取培养 ADSCs，再制备能血管内皮生长因子(vascular endothelial growth factor，VEGF) 缓释聚乳酸-羟基乙酸共聚物纳米微球复合支架(polylactic-co-glycolic acid，PLGA)，将ADSCs与复合支架植入裸小鼠的背部，通过测量植入物的重量、组织切片 HE 染色观察，探讨ADSCs和复合支架移植进行组织工程化脂肪构建的可行性。, http://www.100md.com(王永恒 李林瑞 雷雨)