骨碎补对牙周炎大鼠正畸牙移动保持阶段RANKL表达影响的研究(1)
摘要：目的 研究牙周炎的大鼠牙齒移动保持阶段，中药骨碎补对牙槽骨成骨作用以及对RANKL表达影响的研究。方法 将45只雄性Wister大鼠建立牙周炎实验模型。按照随机数字表法将大鼠分成9个组，每组5只，进行标记。其中1组设为空白组，4组对照组，4组实验组。空白组拉簧牵拉上颌左侧第一磨牙向近中移动，牵拉21 d后分别在保持3，7，14，21 d处死;实验组和对照组分别建立牙移动模型，实验组在保持阶段的3，7，14，21 d的前1天灌服骨碎补水煎液0.9 g/kg， 1次/d;对照组同样时间灌服等量0.9%氯化钠溶液，1次/d。HE染色观察成骨细胞和破骨细胞。免疫组织化学切片观察RANKL因子表达量。结果 实验组的RANKL表达量在初期先增加，从第7 d开始RANKL表达量开始逐渐降低。同一节点对比，实验组RANKL表达量低于对照组。实验组在保持第21 d RANKL表达量非常少，牙周膜处于稳定状态，差异有统计学意义(P<0.05)。结论 骨碎补水煎液可以促进成骨，稳定牙槽骨及牙周膜的改建，降低RANKL的表达。
关键词：骨碎补;牙周炎;牙移动;RANKL
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中图分类号：R781.4 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.028
文章编号：1006-1959(2019)04-0085-03
Abstract：Objective To study the effect of Chinese medicine granules on the alveolar bone osteogenesis and the expression of RANKL in periodontitis rats. Methods 45 male Wister rats were established experimental models of periodontitis. Rats were divided into 9 groups according to the random number table method， and 5 rats in each group were labeled. One group was set as blank group， 4 groups were control group， and 4 groups were experimental group. The blank group was pulled to pull the first molar on the left side of the upper jaw and moved to the middle. After 21 d of pulling， the rats were sacrificed for 3， 7， 14 and 21 d.The tooth movement model was established in the experimental group and the control group respectively.The experimental group was given 0.9 g/kg of decoction of bone decoction once a day， 3， 7， 14， 21 d of the maintenance phase， once a day;The control group was given the same amount of 0.9% sodium chloride solution once a day for the same time. Osteoblasts were observed by HE staining. Immunohistochemical sections were used to observe the expression level of RANKL factor. Results The expression level of RANKL in the experimental group increased first， and the expression level of RANKL began to decrease gradually from the 7th day. Compared with the same node， the expression level of RANKL in the experimental group was lower than that in the control group. In the experimental group， the expression of RANKL was very low at 21 d and the periodontal ligament was stable，the difference was statistically significant (P<0.05). Conclusion Wound decoction can promote osteogenesis， stabilize the alveolar bone and periodontal ligament， and reduce the expression of RANKL., 百拇医药(宋佳 赵刚 宋春蕾)
关键词：骨碎补;牙周炎;牙移动;RANKL
, http://www.100md.com
中图分类号：R781.4 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.028
文章编号：1006-1959(2019)04-0085-03
Abstract：Objective To study the effect of Chinese medicine granules on the alveolar bone osteogenesis and the expression of RANKL in periodontitis rats. Methods 45 male Wister rats were established experimental models of periodontitis. Rats were divided into 9 groups according to the random number table method， and 5 rats in each group were labeled. One group was set as blank group， 4 groups were control group， and 4 groups were experimental group. The blank group was pulled to pull the first molar on the left side of the upper jaw and moved to the middle. After 21 d of pulling， the rats were sacrificed for 3， 7， 14 and 21 d.The tooth movement model was established in the experimental group and the control group respectively.The experimental group was given 0.9 g/kg of decoction of bone decoction once a day， 3， 7， 14， 21 d of the maintenance phase， once a day;The control group was given the same amount of 0.9% sodium chloride solution once a day for the same time. Osteoblasts were observed by HE staining. Immunohistochemical sections were used to observe the expression level of RANKL factor. Results The expression level of RANKL in the experimental group increased first， and the expression level of RANKL began to decrease gradually from the 7th day. Compared with the same node， the expression level of RANKL in the experimental group was lower than that in the control group. In the experimental group， the expression of RANKL was very low at 21 d and the periodontal ligament was stable，the difference was statistically significant (P<0.05). Conclusion Wound decoction can promote osteogenesis， stabilize the alveolar bone and periodontal ligament， and reduce the expression of RANKL., 百拇医药(宋佳 赵刚 宋春蕾)