干扰素-γ对乳腺癌细胞HLA-Ⅰ分子表达的影响(1)
摘要：目的 研究干扰素-γ(IFN-γ)对乳腺癌细胞表面HLA-Ⅰ类分子表达的影响，并探讨其在乳腺癌治疗中的免疫作用机制。方法 培养乳腺癌SKBR3细胞株，根据IFN-γ浓度将其分为25 U/ml、50 U/ml、100 U/ml组，未加药组为空白对照组。通过实时荧光定量PCR和流式细胞术实验检测经不同浓度IFN-γ作用后的乳腺癌SKBR3细胞内HLA-Ⅰ mRNA的含量，以及表面的HLA-Ⅰ类抗原的表达。结果 实时荧光定量PCR结果显示，空白对照组、25 U/ml、50 U/ml、100 U/ml组HLA-Ⅰ mRNA的表达量分别为(1.00±0.00)、(2.26±0.54)、(3.89±0.81)、(5.94±0.97)，三个浓度组均较空白对照组表达量高(P<0.05);流式细胞术结果显示，空白对照组、25 U/ml、50 U/ml、100 U/ml组细胞表面HLA-Ⅰ蛋白表达量为(14.87±2.80)、(44.33±3.36)、(48.80±2.63)、(56.77±1.93)，三个浓度组均高于对照组(P<0.05)。结论 IFN-γ上调乳腺癌细胞HLA-Ⅰ类抗原的表达有利于抗乳腺癌作用。
, http://www.100md.com
关键词：干扰素-γ;乳腺癌;HLA;免疫治疗
中图分类号：R737.9 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.029
文章编号：1006-1959(2019)04-0088-03
Abstract：Objective To investigate the effect of interferon-γ (IFN-γ) on the expression of HLA class Ⅰ molecules on breast cancer cells and to explore its immune mechanism in the treatment of breast cancer. Methods Breast cancer SKBR3 cell line was cultured and divided into 25 U/ml，50 U/ml and 100 U/ml groups according to IFN-γ concentration. The untreated group was a blank control group. Real-time quantitative PCR and flow cytometry were used to detect the content of HLA-Ⅰ mRNA and the expression of HLA-Ⅰ antigen on the surface of breast cancer SKBR3 cells treated with different concentrations of IFN-γ. Results The real-time PCR results showed that the expression levels of HLA-Ⅰ mRNA in the blank control group， 25 U/ml， 50 U/ml and 100 U/ml were (1.00±0.00)，(2.26±0.54)，(3.89±0.81)，(5.94±0.97)，the three concentration groups were higher than the blank control group (P<0.05); the flow cytometry results showed that the blank control group， 25 U/ml， 50 U/ml The expression of HLA-Ⅰ protein in the cell surface of the 100 U/ml group was (14.87±2.80)， (44.33±3.36)， (48.80±2.63)， (56.77±1.93)， and the three concentration groups were higher than the control group (P<0.05). Conclusion IFN-γ up-regulates the expression of HLA-Ⅰ antigen in breast cancer cells， which is beneficial to the anti-breast cancer.
, 百拇医药
Key words：Interferon-γ;Breast cancer;HLA;Immunotherapy
乳腺癌(Breast cancer)是目前世界上第2大常見的恶性肿瘤，位居女性恶性肿瘤第1位。发病率占成人恶性肿瘤的3%，仅次于肺癌。2018年新增的乳腺癌病例超过200万例[1]。尽管乳腺癌的早期筛查、手术治疗、放化疗、内分泌治疗以及分子靶向治疗已取得重大进展，但乳腺癌仍然是女性肿瘤死亡的重大疾病[2]。肿瘤免疫理论认为免疫逃逸机制在肿瘤的发生和发展过程中起着关键作用。近年来，免疫逃避已被公认为癌症进展的标志[3]。MHC(人类为HLA)-Ⅰ类分子表达缺陷是肿瘤免疫逃逸的重要机制。研究发现IFN-γ可以上调多种肿瘤细胞表面MHC-Ⅰ类分子的表达，有利于阻断肿瘤免疫逃逸。但对乳腺癌细胞表面MHC-Ⅰ类分子表达的作用有待进一步研究。本研究应用低表达HLA-I的乳腺癌细胞探索IFN-γ对其细胞表面HLA-Ⅰ类分子表达的上调。, 百拇医药(刘丽 孙立新 赵明珍)
, http://www.100md.com
关键词：干扰素-γ;乳腺癌;HLA;免疫治疗
中图分类号：R737.9 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.029
文章编号：1006-1959(2019)04-0088-03
Abstract：Objective To investigate the effect of interferon-γ (IFN-γ) on the expression of HLA class Ⅰ molecules on breast cancer cells and to explore its immune mechanism in the treatment of breast cancer. Methods Breast cancer SKBR3 cell line was cultured and divided into 25 U/ml，50 U/ml and 100 U/ml groups according to IFN-γ concentration. The untreated group was a blank control group. Real-time quantitative PCR and flow cytometry were used to detect the content of HLA-Ⅰ mRNA and the expression of HLA-Ⅰ antigen on the surface of breast cancer SKBR3 cells treated with different concentrations of IFN-γ. Results The real-time PCR results showed that the expression levels of HLA-Ⅰ mRNA in the blank control group， 25 U/ml， 50 U/ml and 100 U/ml were (1.00±0.00)，(2.26±0.54)，(3.89±0.81)，(5.94±0.97)，the three concentration groups were higher than the blank control group (P<0.05); the flow cytometry results showed that the blank control group， 25 U/ml， 50 U/ml The expression of HLA-Ⅰ protein in the cell surface of the 100 U/ml group was (14.87±2.80)， (44.33±3.36)， (48.80±2.63)， (56.77±1.93)， and the three concentration groups were higher than the control group (P<0.05). Conclusion IFN-γ up-regulates the expression of HLA-Ⅰ antigen in breast cancer cells， which is beneficial to the anti-breast cancer.
, 百拇医药
Key words：Interferon-γ;Breast cancer;HLA;Immunotherapy
乳腺癌(Breast cancer)是目前世界上第2大常見的恶性肿瘤，位居女性恶性肿瘤第1位。发病率占成人恶性肿瘤的3%，仅次于肺癌。2018年新增的乳腺癌病例超过200万例[1]。尽管乳腺癌的早期筛查、手术治疗、放化疗、内分泌治疗以及分子靶向治疗已取得重大进展，但乳腺癌仍然是女性肿瘤死亡的重大疾病[2]。肿瘤免疫理论认为免疫逃逸机制在肿瘤的发生和发展过程中起着关键作用。近年来，免疫逃避已被公认为癌症进展的标志[3]。MHC(人类为HLA)-Ⅰ类分子表达缺陷是肿瘤免疫逃逸的重要机制。研究发现IFN-γ可以上调多种肿瘤细胞表面MHC-Ⅰ类分子的表达，有利于阻断肿瘤免疫逃逸。但对乳腺癌细胞表面MHC-Ⅰ类分子表达的作用有待进一步研究。本研究应用低表达HLA-I的乳腺癌细胞探索IFN-γ对其细胞表面HLA-Ⅰ类分子表达的上调。, 百拇医药(刘丽 孙立新 赵明珍)