可手术的非小细胞肺癌患者外周静脉血cfDNA总浓度及长片段DNA浓度的临床价值研究(1)
摘要：目的 探讨定量检测可手术的非小细胞肺癌患者外周血cfDNA总浓度及长片段DNA浓度的临床价值。方法 选择2017年1月～12月承德市中心医院胸外科60例可手术的非小细胞肺癌患者为实验组，分别于手术前1 d、手术后7 d、手术后20 d采集外周静脉血液标本，同期于承德市中心医院体检中心选择60例健康体检者为对照组，采集外周静脉血液标本，定量检测两组标本血液中cfDNA总浓度及长片段DNA浓度，进行统计学分析。结果 实验组患者手术前1 d外周静脉血中cfDNA总浓度较对照组升高，差异具有统计学意义(P<0.05);实验组患者术后7 d外周静脉血中cfDNA总浓度较术前1 d无明显变化，差异无统计学意义(P>0.05);实验组患者术后20 d外周静脉血中cfDNA总浓度较术前1 d、术后7 d降低，差异均有统计学意义(P<0.05)。实验组患者手术前1 d外周静脉血中长片段DNA浓度高于对照组，差异具有统计学意义(P<0.05);实验组患者术后7 d外周静脉血中长片段DNA浓度较术前1 d无明显变化，差异无统计学意义(P>0.05);实验组患者术后20 d外周静脉血中长片段DNA浓度较术前1 d、术后7 d降低，差异均有统计学意义(P<0.05)。结论 外周静脉血cfDNA总浓度及长片段DNA浓度可间接反映非小细胞肺癌患者肿瘤负荷状况，有望成为非小细胞肺癌辅助诊断、疗效评估及预后判断的参考指标。
, 百拇医药
关键词：非小细胞肺癌;外周血cfDNA总浓度;长片段DNA濃度
中图分类号：R734.2 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.030
文章编号：1006-1959(2019)04-0091-04
Abstract：Objective To investigate the clinical value of quantitative detection of total cfDNA concentration and long DNA concentration in peripheral blood of patients with operable non-small cell lung cancer. Methods From January to December 2017， 60 patients with operable non-small cell lung cancer in the Department of Thoracic Surgery， Chengde Central Hospital were selected as experimental group. Peripheral venous blood samples were collected 1 d before surgery， 7 d after surgery and 20 d after surgery. In the same period， 60 healthy people in the physical examination center of Chengde Central Hospital were selected as the control group. Peripheral venous blood samples were collected， and the total concentration of cfDNA and long DNA concentration in the blood of the two groups were quantitatively analyzed for statistical analysis. Results The total concentration of cfDNA in peripheral vein blood in the experimental group was significantly higher than that in the control group one day before operation，the difference was statistically significant (P<0.05)，There was no significant change in the total concentration of cfDNA in the peripheral venous blood of the patients in the experimental group at 7 d after operation compared with that in the first day before operation (P>0.05)，the total concentration of cfDNA in the peripheral venous blood of the patients in the experimental group was significantly lower than that in the first day of operation and 7 d after operation (P<0.05) at the 20th d after operation，The concentration of long fragment DNA in peripheral venous blood of the experimental group was higher than that of the control group 1 d before operation，the difference was statistically significant (P<0.05).There was no significant difference in the concentration of long-segment DNA in peripheral blood of the experimental group on the 7th d after operation (P>0.05). The long-term DNA concentration in the peripheral venous blood of the experimental group was 20 d after operation. Compared with 1 d before surgery and 7 d after operation， the difference was statistically significant (P<0.05). Conclusion The total concentration of cfDNA and long-segment DNA concentration in peripheral venous blood can indirectly reflect the tumor burden of non-small cell lung cancer patients， and it is expected to be a reference index for auxiliary diagnosis， efficacy evaluation and prognosis of non-small cell lung cancer., 百拇医药(贾殿军 王利军 程险峰)
, 百拇医药
关键词：非小细胞肺癌;外周血cfDNA总浓度;长片段DNA濃度
中图分类号：R734.2 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.030
文章编号：1006-1959(2019)04-0091-04
Abstract：Objective To investigate the clinical value of quantitative detection of total cfDNA concentration and long DNA concentration in peripheral blood of patients with operable non-small cell lung cancer. Methods From January to December 2017， 60 patients with operable non-small cell lung cancer in the Department of Thoracic Surgery， Chengde Central Hospital were selected as experimental group. Peripheral venous blood samples were collected 1 d before surgery， 7 d after surgery and 20 d after surgery. In the same period， 60 healthy people in the physical examination center of Chengde Central Hospital were selected as the control group. Peripheral venous blood samples were collected， and the total concentration of cfDNA and long DNA concentration in the blood of the two groups were quantitatively analyzed for statistical analysis. Results The total concentration of cfDNA in peripheral vein blood in the experimental group was significantly higher than that in the control group one day before operation，the difference was statistically significant (P<0.05)，There was no significant change in the total concentration of cfDNA in the peripheral venous blood of the patients in the experimental group at 7 d after operation compared with that in the first day before operation (P>0.05)，the total concentration of cfDNA in the peripheral venous blood of the patients in the experimental group was significantly lower than that in the first day of operation and 7 d after operation (P<0.05) at the 20th d after operation，The concentration of long fragment DNA in peripheral venous blood of the experimental group was higher than that of the control group 1 d before operation，the difference was statistically significant (P<0.05).There was no significant difference in the concentration of long-segment DNA in peripheral blood of the experimental group on the 7th d after operation (P>0.05). The long-term DNA concentration in the peripheral venous blood of the experimental group was 20 d after operation. Compared with 1 d before surgery and 7 d after operation， the difference was statistically significant (P<0.05). Conclusion The total concentration of cfDNA and long-segment DNA concentration in peripheral venous blood can indirectly reflect the tumor burden of non-small cell lung cancer patients， and it is expected to be a reference index for auxiliary diagnosis， efficacy evaluation and prognosis of non-small cell lung cancer., 百拇医药(贾殿军 王利军 程险峰)