食管鳞癌组织中miRNAs差异表达谱的筛选及研究(1)
摘要：目的 筛选并鉴定在食管鳞癌(ESCC)及癌旁组织中miRNAs的差异表达，为进一步阐明其在ESCC发病机制中的作用奠定基础。方法 选取在邯郸市第一医院行食管癌切除术患者新鲜的鳞癌组织及癌旁正常组织，各3例，抽提总RNA，利用miRNAs芯片筛选其中差异表达的miRNAs，并对miR-106b-3p通过进一步RT-qPCR 技术进行验证。结果 miRNAs芯片從配对组织中共筛检出62个差异表达的miRNAs，其中41个miRNAs表达上调，21个miRNAs表达下调，鳞癌组织与癌旁正常组织中差异表达的miRNAs比较，差异有统计学意义(P<0.05)。miR-106b-3p在ESCC组织中的表达高于癌旁组织，差异有统计学意义(P<0.05)，与芯片结果一致。结论 食管鳞癌组织中miRNAs的差异表达为进一步研究miRNAs在ESCC发病中的作用奠定了基础;miR-106b-3p的高表达可能与ESCC的发生、发展有关。
关键词：食管鳞癌;miRNAs芯片;microRNAs
中图分类号：R735.1 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.20.018
文章编号：1006-1959(2019)20-0063-04
Screening and Study of Differential Expression Profiles of miRNAs in
Esophageal Squamous Cell Carcinoma
YANG Ya-juan1，LI Jing1，ZHU Lin2，SHI Jun-jie3，QIAO Guan-en1
Abstract：Objective To screen and identify differential expression of miRNAs in esophageal squamous cell carcinoma (ESCC) and adjacent tissues， and to lay the foundation for further elucidation of its role in the pathogenesis of ESCC.Methods Fresh squamous cell carcinoma and adjacent normal tissues were obtained from patients with esophageal cancer resection in the First Hospital of Handan City， 3 cases were extracted， total RNA was extracted， and miRNAs were used to screen differentially expressed miRNAs， and miR-106b-3p was used. Verification by further RT-qPCR technology.Results A total of 62 differentially expressed miRNAs were screened from the paired tissues by miRNAs. Among them， 41 miRNAs were up-regulated， 21 miRNAs were down-regulated， and miRNAs were differentially expressed in squamous cell carcinoma and adjacent normal tissues，the difference was statistically significant (P<0.05). The expression of miR-106b-3p in ESCC tissues was higher than that in adjacent tissues，the difference was statistically significant (P<0.05)， which was consistent with the chip results.Conclusion The differential expression of miRNAs in esophageal squamous cell carcinoma lays a foundation for further study of the role of miRNAs in the pathogenesis of ESCC. The high expression of miR-106b-3p may be related to the occurrence and development of ESCC.
Key words：Esophageal squamous cell carcinoma;miRNAs chip;microRNAs
食管鳞癌(esophageal squamous cell carcinoma，ESCC)是我国常见的消化道恶性肿瘤，其发病的机制尚不清楚。microRNAs(miRNAs)是一类长度在22 nt左右的非编码小RNAs，参与了人体内大部分生物学调节途径[1，2]。miRNAs能够通过其碱基序列区域与其相应靶基因mRNA的3'-非翻译区(3'-untranslated region，3'-UTR)中的互补碱基序列进行完全或不完全的互补结合，在基因的转录后阶段调控其相应靶基因mRNA的表达水平[3-5]。miRNAs芯片又称microRNAs微阵列，可同时检测数千个miRNAs的表达量，适用于miRNAs表达谱分析。本研究采用安捷伦miRNAs芯片技术筛选ESCC组织与癌旁正常组织差异表达的miRNAs，并对部分miRNA采用反转录荧光定量PCR(RT-qPCR)进行验证，为进一步研究miRNAs在ESCC发生、发展过程中的作用提供参考。, http://www.100md.com(杨雅娟 李静 朱琳 石俊杰 乔冠恩)
关键词：食管鳞癌;miRNAs芯片;microRNAs
中图分类号：R735.1 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.20.018
文章编号：1006-1959(2019)20-0063-04
Screening and Study of Differential Expression Profiles of miRNAs in
Esophageal Squamous Cell Carcinoma
YANG Ya-juan1，LI Jing1，ZHU Lin2，SHI Jun-jie3，QIAO Guan-en1
Abstract：Objective To screen and identify differential expression of miRNAs in esophageal squamous cell carcinoma (ESCC) and adjacent tissues， and to lay the foundation for further elucidation of its role in the pathogenesis of ESCC.Methods Fresh squamous cell carcinoma and adjacent normal tissues were obtained from patients with esophageal cancer resection in the First Hospital of Handan City， 3 cases were extracted， total RNA was extracted， and miRNAs were used to screen differentially expressed miRNAs， and miR-106b-3p was used. Verification by further RT-qPCR technology.Results A total of 62 differentially expressed miRNAs were screened from the paired tissues by miRNAs. Among them， 41 miRNAs were up-regulated， 21 miRNAs were down-regulated， and miRNAs were differentially expressed in squamous cell carcinoma and adjacent normal tissues，the difference was statistically significant (P<0.05). The expression of miR-106b-3p in ESCC tissues was higher than that in adjacent tissues，the difference was statistically significant (P<0.05)， which was consistent with the chip results.Conclusion The differential expression of miRNAs in esophageal squamous cell carcinoma lays a foundation for further study of the role of miRNAs in the pathogenesis of ESCC. The high expression of miR-106b-3p may be related to the occurrence and development of ESCC.
Key words：Esophageal squamous cell carcinoma;miRNAs chip;microRNAs
食管鳞癌(esophageal squamous cell carcinoma，ESCC)是我国常见的消化道恶性肿瘤，其发病的机制尚不清楚。microRNAs(miRNAs)是一类长度在22 nt左右的非编码小RNAs，参与了人体内大部分生物学调节途径[1，2]。miRNAs能够通过其碱基序列区域与其相应靶基因mRNA的3'-非翻译区(3'-untranslated region，3'-UTR)中的互补碱基序列进行完全或不完全的互补结合，在基因的转录后阶段调控其相应靶基因mRNA的表达水平[3-5]。miRNAs芯片又称microRNAs微阵列，可同时检测数千个miRNAs的表达量，适用于miRNAs表达谱分析。本研究采用安捷伦miRNAs芯片技术筛选ESCC组织与癌旁正常组织差异表达的miRNAs，并对部分miRNA采用反转录荧光定量PCR(RT-qPCR)进行验证，为进一步研究miRNAs在ESCC发生、发展过程中的作用提供参考。, http://www.100md.com(杨雅娟 李静 朱琳 石俊杰 乔冠恩)