HPLC法测定癌痛消方及其拆方中松脂醇二葡萄糖苷和芍药苷的含量(1)
摘要：目的 建立癌痛消方及其拆方中松脂醇二葡萄糖苷和芍藥苷的测定方法。方法 采用十八烷基硅烷键合硅胶柱(Inertsil ODS-3柱 250×4.6 mm，5 μm);流动相：乙腈-0.1%磷酸(15∶85)为流动相，进行梯度洗脱;检测波长：230 nm(0～16 min，松脂醇二葡萄糖苷)，277 nm(16～30 min，芍药苷);流速：1.0 ml/min;柱温：30oC。结果 癌痛消-补虚组中松脂醇二葡萄糖苷在1.0～5.0 μg范围内呈现良好的线性关系，r=0.9992，平均回收率为97.23%(RSD=1.31%，n=6);癌痛消-抑癌组中芍药苷在1.0～5.0 μg范围内呈现良好的线性关系，r=0.9993，平均回收率为97.08%(RSD=1.38%，n=6);癌痛消-全方中松脂醇二葡萄糖苷在1.0～5.0 μg范围内呈现良好的线性关系，r=0.9992，平均回收率为98.01%(RSD=1.30%，n=6);癌痛消-全方中芍药苷在1.0～5.0μg范围内呈现良好的线性关系，r=0.9993，平均回收率为96.97%(RSD=1.54%，n=6);结论 本方法专属性强，准确度高，重现性良好，可用于癌痛消方的质量控制。
关键词：癌痛消方;含量测定;松脂醇二葡萄糖苷;芍药苷;HPLC
中图分类号：R286.0 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.23.020
文章编号：1006-1959(2019)23-0073-05
Determination of Rosinol Diglucoside and Paeoniflorin in Cancer Painkiller and
Its Decomposed Formula by HPLC
CHENG Jin-Lai，LIANG Dan，XIA Meng，CAO Zi-Yin，LENG Jing
(Basic Medical College of Guangxi University of Traditional Chinese Medicine/Guangxi Key Laboratory of Laboratory Animal Disease Models/Guangxi Key Laboratory of Integrated Traditional Chinese and Western Medicine and Translational Medicine
for High Infectious Diseases，Nanning 530200，Guangxi，China)
Abstract：Objective To establish a method for the determination of rosinol diglucoside and paeoniflorin in Cancer Decoction and its decomposed formula. Methods An octadecylsilane bonded silica gel column (Inertsil ODS-3 column 250×4.6 mm， 5 μm); mobile phase： acetonitrile-0.1% phosphoric acid (15：85) as mobile phase， gradient elution; detection wavelength： 230 nm (0～16 min， rosinol diglucoside)， 277 nm (16～30 min， paeoniflorin); flow rate： 1.0 ml/min; column temperature： 30oC. Results In the cancer pain-compensation group， rosinol diglucoside showed a good linear relationship in the range of 1.0～5.0 μg， r=0.9992， and the average recovery was 97.23% (RSD=1.31%， n=6);The paeoniflorin showed a good linear relationship in the range of 1.0～5.0 μg， r=0.9993， and the average recovery was 97.08% (RSD=1.38%， n=6). Cancer pain elimination-total rosin Diglucoside showed a good linear relationship in the range of 1.0～5.0 μg， r=0.9992， the average recovery was 98.01% (RSD=1.30%， n=6); The cancer has a good linear relationship in the range of 1.0～5.0μg， r=0.9993， and the average recovery rate is 96.97% (RSD=1.54%， n=6). Conclusion This method has strong specificity. High accuracy and good reproducibility， can be used for quality control of cancer pain., http://www.100md.com(程金来 梁丹 夏猛 曹子茵 冷静)
关键词：癌痛消方;含量测定;松脂醇二葡萄糖苷;芍药苷;HPLC
中图分类号：R286.0 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.23.020
文章编号：1006-1959(2019)23-0073-05
Determination of Rosinol Diglucoside and Paeoniflorin in Cancer Painkiller and
Its Decomposed Formula by HPLC
CHENG Jin-Lai，LIANG Dan，XIA Meng，CAO Zi-Yin，LENG Jing
(Basic Medical College of Guangxi University of Traditional Chinese Medicine/Guangxi Key Laboratory of Laboratory Animal Disease Models/Guangxi Key Laboratory of Integrated Traditional Chinese and Western Medicine and Translational Medicine
for High Infectious Diseases，Nanning 530200，Guangxi，China)
Abstract：Objective To establish a method for the determination of rosinol diglucoside and paeoniflorin in Cancer Decoction and its decomposed formula. Methods An octadecylsilane bonded silica gel column (Inertsil ODS-3 column 250×4.6 mm， 5 μm); mobile phase： acetonitrile-0.1% phosphoric acid (15：85) as mobile phase， gradient elution; detection wavelength： 230 nm (0～16 min， rosinol diglucoside)， 277 nm (16～30 min， paeoniflorin); flow rate： 1.0 ml/min; column temperature： 30oC. Results In the cancer pain-compensation group， rosinol diglucoside showed a good linear relationship in the range of 1.0～5.0 μg， r=0.9992， and the average recovery was 97.23% (RSD=1.31%， n=6);The paeoniflorin showed a good linear relationship in the range of 1.0～5.0 μg， r=0.9993， and the average recovery was 97.08% (RSD=1.38%， n=6). Cancer pain elimination-total rosin Diglucoside showed a good linear relationship in the range of 1.0～5.0 μg， r=0.9992， the average recovery was 98.01% (RSD=1.30%， n=6); The cancer has a good linear relationship in the range of 1.0～5.0μg， r=0.9993， and the average recovery rate is 96.97% (RSD=1.54%， n=6). Conclusion This method has strong specificity. High accuracy and good reproducibility， can be used for quality control of cancer pain., http://www.100md.com(程金来 梁丹 夏猛 曹子茵 冷静)