Sub-acute Toxicity of Carbofuran on Acetylcholines

http://www.100md.com 2005年10月1日 Rajendra K Singh B. Sharma

Sub-acuteToxicityofCarbofuranonAcetylcholinesteraseActivityintheFreshwaterCatfish

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     Abstract: The inhibition of acetylcholinesterase (ACHE) activity has been widely used as a biomarker in an animal exposed tothe pesticides. However, the interaction of extensively used organocarbamate insecticide, carbofuran, with the nervous system of theaquatic organisms is not properly studied. AChE is a key enzyme which catalyses the hydrolysis of acetylcholine, a neurotransmitterat the neuromuscular junctions, and thus regulates the neurotransmission system. In the present study, we have evaluated the impactof sub-acute concentrations (0.01 and 0.02 mg/L i.e. 1/20^th and 1/10^th of LC₅₀) of carbofuran on the activity of acetylcholinesterase,from different tissues of Clarias batrachus, a fresh water teleost, after 96 hr and 15 days exposure periods in vivo. The carbofuransignificantly reduced the activity of AChE in different tissues of C. batrachus at both concentrations and periods of exposure. Thegreater inhibition of AChE activities were recorded in fish tissues at higher carbofuran concentration (0.02 mg/L) after longer (15days) treatment period. The inhibition of AChE activity in all fish tissues tested was dependent on pesticide concentration and theduration of treatment. AChE from the tissues of C. batrachus was found to be a true cholinesterase as it was completely inhibited bythe small concentration (nM) of eserine as tested in vitro. It was found that carbofuran at very low concentration exerted significantinhibitory effect on AChE activity in fish tissues.

    Key Words: carbofuran; Clariasbatrachus; toxicity; acetylcholinesterase; inhibition

    Article ID:1006-3617(2005)05-0403-05

    CLC Number:R114

    Document Code:A

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