Uterotrophic and Hershberber Assays for Acrylonitr
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Abstract: [ Objective ] To assess the estrogenic and anti-androgenic effects of acrylonitrile (AN) by in-vivo uterotrophicand Hershberger assays proposed by the OECD. [ Methods ] For the uterotrophic assay, AN was injected subcutaneously atthe doses of 0, 10, 15, 25, 40 and 60mg/kg to 56-day-old ovariectomized rats for 3 days. In some rats, 17β-estradiol (EE) was alsoinjected subcutaneously at a dose of 0.001 mg/kg as a positive control group. For the Hershberger assay, AN was administered orallyat doses of 0, 10, 20, 30, 40 and 50 mg/kg to 56-day-old castrated rats for 10 days. 0.4 mg/kg testosterone propionate (TP) was alsoadministered by subcutaneous injection after the administration of AN. In some rats, flutamide (FLU) was also administered orally ata dose of 25 mg/kg as the reference chemical for anti-vandrogenicity. [ Results ] For the uterotrophic assay, there were essentiallyno differences in the uterine wet or blotted weights between the control and any of the AN-treated groups. For the Hershberger assay,the weights of the accessory sex organs of the castrated rats showed no significant differences between the oil plus TP-treated groupand any of the AN plus TP-treated groups. [ Conclusion ] These findings suggest that AN dose not have any endocrine-disruptingproperties in in-vivo screening tests.
Key Words: acrylonitrile: uterotrophic assay: Hershberger assay: 171β-estradioi: testosterone propionate:endocrine disruptor
Article ID: 1006-3617(2005)05-0408-05
CLC Number:R114
Document Code:A
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