苦参碱对人增生性瘢痕成纤维细胞增殖的影响

http://www.100md.com 2001年2月1日《中国美容医学》 2001年第1期

     汤苏阳女，1964年生，1987年毕业于锦州医学院医疗系，1998期部队中、青年人才基金班毕业，医学硕士。发表论文十余篇，参编专著一部。[摘要]目的：研究苦参碱(Matrine,简称Mat)对人增生性瘢痕成纤维细胞增殖的影响。方法：将Mat以不同浓度梯度作用于体外培养的增生性瘢痕成纤维细胞，测MTT反应的A0值及LDH值，用免疫组织化学检测Mat用药前后的细胞核内增殖性抗原(proliferating cell nuclear antigen,PCNA)的表达。结果：增生性瘢痕成纤维细胞在Mat0.25～100mg/ml范围内呈剂量依赖性增殖抑制，但LDH值无明显改变；PCNA的表达，在Mat作用后明显减弱。结论：Mat在体外能明显抑制增生性瘢痕成纤维细胞的增殖而不引起细胞坏死性改变。

    [关键词]苦参碱成纤维细胞增殖

    [中图分类号]R619+.6[文献标识码]A[文章编号]1008-6455(2001)01-0006-03

    THE INFLUENCE OF MATRINE ON THE GROWTH AND PROLIFERATION

    OF SCAR-DERIVED FIBROBLASTS

    TANG Su-yangCAI Bao-renHUANG Gao-sheng

    Department of burn and plastic,205 Hospital of PLA(Jin Zhou 121001)china

    [Abstract]Objective：To study the influence of Matrine on growth and proliferation of fibroblasts derived hgpertropnic scar.〖WT5"HZ〗Methods：The MTT OD value and the content of LDH was examined by biochemical analysis after Matrine was added to the monolayer culture system of scar-derived fibroblasts.The expressions of prolifrating cell nuclear antigen (PCNA)were examinedby immunochemied methed.Results：Mat at 0.10～100.mg/ml could decrease the MTT OD value in dose dependent manner amd could not increase the content of LDH significantly.The expression of PCNA in Mat groupswas highr than the control groups.Conclusion：Mat could inhibit the proliferation of derived hypertropnicscar fibroblasts rarely produce the toxicity on scar-derived fibroblasts. ......

百拇医药网 http://www.100md.com/html/paper/1008-6455/2001/01/02.htm

您现在查看是摘要页，全文长 6804 字符。