[摘要]目的：探讨犬乳恒牙替换过程中骨保护素在犬恒牙胚发育中的表达及意义。方法：分别对乳牙期、替牙期、恒牙期本地犬取牙胚及其周围组织,制备石蜡切片,进行HE染色和牙胚内OPG的免疫组化染色,用彩色病理图像分析系统分析各组染色强度灰度值。结果：乳牙期、替牙期犬牙胚的成牙本质细胞OPG的免疫组化强度明显高于另外一组,成釉细胞中也有阳性信号。结论：OPG可能参与恒牙胚的形成和发育。

    [关键词]犬恒牙胚；骨保护素；成牙本质细胞；成釉细胞

    [中图分类号]R781 [文献标识码]A [文章编号]1008-6455(2008)06-0877-2

    The expression and significance of osteoprotegerin in the the development of of dogs' permanent teeth germs

    CHEN Li-ping1，WU Wei1，BAI Hui-ying1，YANG Fu-sheng2

    (1.Department of Dentistry, Qinghai Provincial Corps Hospital,Chinese Pepole′s Armed Police Force, Xi′ning 810001,Qinghai,China;2.College of Stomatology,Fourth Military Medical University)

    Abstract:ObjectiveTo study the expression and significance of osteoprotegerin(OPG)in the development of dogs'permanent teeth germs. MethodsDogs of different ages were selected as objects,selected permanent teeth germs and their peripheral tissue of dogs in different periods of primary dentition roots stability ......