组织工程化犬气管的生物力学研究(1)
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[摘要]目的:通过检测不同方法处理的脱细胞犬气管基质在无载荷及零应力状态下的张开角和残余应变,并对组织工程化气管的力学性能进行初步探讨。方法:取犬的第5～10气管环,分别进行酶消化法和深低温冷冻法处理,测量制成的脱细胞犬气管基质与新鲜气管在无载荷及零应力下的张开角及残余应变。结果:新鲜犬气管的张开角大于2组脱细胞犬气管基质的张开角,其差异具有统计学意义。酶消化法脱细胞犬气管基质的张开角及残余应变大于深低温冷冻组,其差异具有统计学意义。结论:不同方法脱细胞处理后,犬气管的径向支撑性能将受到影响,其中酶消化法构建的组织工程气管基质抗压缩能力优于冷冻法处理组的气管基质。
[关键词]组织工程;犬;气管;生物力学;张开角
[中图分类号]R318 [文献标识码]A [文章编号]1008-6455(2010)07-1015-03
Biomechanical research of tissue engineered canine trachea
ZHANG Wen-hao1,HUO Ran1,LI Shang-bin2,YU Qing-ping3,ZHAO Yang1,GAO Feng1
(1.Department of Cosmetology and Plastic and Burn, Provincial Hospitial Affiliated to Shandong University,Jinan 250021,Shandong, China; 2.Department of Nuclear Medicine, Provincial Hospitial Affiliated to Shandong University; 3. Heze Municipal Hospital of Shandong Province)
Abstract:ObjectiveTo find the basic mechanical properties of tissue engineered trachea by detecting the opening angles and residual strains of acellular matrix of canine tracheas in the no-load and zero-stress states.Methods The 5th～10th tracheal rings below thyroid cartilage of canine were respectively treated with modified Courtman enzyme digest method and cryopreserved method. Then detect the opening angles and residual strains of these two acellular matrix groups and fresh trachea group in the no-load and zero-stress states.ResultsThe opening angles of fresh trachea group were larger than those two acellular matrix groups with statistical difference. The opening angles and residual strains of modified Courtman method group were larger than cryopreserved method group with statistical difference. ConclusionBoth modified Courtman method and cryopreserved method can influencethe radial sustain of canine tracheas, the tissue engineered acellular matrix treated with modified Courtman has a better anti- compressibility than cryopreserved tracheal acellular matrix.
Key words:tissue engineering; canine; trachea; biomechanics; opening angle
各种原因如创伤、肿瘤等引起的长段的气管缺损,因吻合口张力过大而不能直接缝合时,需要用人工气管来修复,其中组织工程化气管因其在免疫排斥、材料来源等方面的优势逐渐成为气管重建外科的研究热点。组织工程化气管较为常用的去抗原方法有深低温冷冻和酶消化法。本实验主要探讨这两种方法处理后的脱细胞气管基质的生物力学性能较新鲜气管有无明显变化,并推测气管移植后的塌陷是否与处理方法的不同造成的力学性能变化有关。
Han[1]和Fung[2]指出零应力气管扇形体虽然不是圆形的,但是利用扇形体内壁中点与内壁两端点连线的夹角所定义的张开角依然可用来描述气管扇形体的主要特征 ......
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