IL-1A基因多态性与深圳市人群牙周炎的相关性研究(1)
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[摘要]目的：探讨IL-1A-889的基因多态性与慢性牙周炎及侵袭性牙周炎易感性的相关关系。材料和方法：选取华南地区慢性牙周炎患者57例，侵袭性牙周炎患者45例，健康志愿者50例，从颊黏膜脱落细胞中获得DNA，用PCR-RFLP法检测IL-1A-889基因型的分布。结果：慢性牙周炎患者的IL-1A-889基因型的分布以及T等位基因的出现频率显著高于对照组，侵袭性牙周炎患者与对照组相比无显著性差异。结论：IL-1A-889基因多态性与深圳市慢性牙周炎患者的易感性有显著相关性。
[关键词]IL-1；基因多态性；慢性牙周炎；侵袭性牙周炎
[中图分类号]R78 [文献标识码]A[文章编号]1008-6455(2011)01-0071-03
Study on the association of IL1A gene polymorphism with periodontitis in Shenzhen individuals
LIU Jian-lin,ZHAO Zhong-cheng
(Department of Stomatology, The Second TCM Hospital of Shenzhen，Shenzhen，518034 Guangdong,China)
Abstract:ObjectiveTo discuss the association of the IL1A (-889) gene polymorphism in Shenzhen individuals with chronic and aggressive periodontitis.Material and methodsDNA was obtained from oral swabs of 152 shenzhen individuals and was amplified using the polymerase chain reaction (PCR). Products were submitted to digestion and were analyzed by electrophoresis to distinguish the C and T alleles through Restriction Fragment Length Polymorphism method (RFLP).Results A significant difference in the genotype distribution was observed when comparing the chronic periodontitis group with the control group. Moreover, a higher incidence of the T allele in the chronic periodontitis group was detected when compared with the control group. No statistical difference was observed when the aggressive periodontitis group was compared with the control group.Conclusion These data show an association of the IL1A (-889) polymorphism with chronic periodontitis in Shenzhen individuals.
Key words:IL-1;gene polymorphism;chronic periodontitis;aggressive periodontitis
牙周炎是一种常见的发生在牙周支持组织的感染性疾病，不同的个体对牙周炎的易感性不同。近年来，牙周炎敏感性相关基因的研究成为热点，越来越多的研究证明IL-1基因多态性与牙周炎敏感性、牙齿缺失等的相关性最为密切[1-5]。本研究拟通过检测IL-1A-889的基因多态性在华南地区牙周炎患者中的分布，观察IL-1基因多态性对慢性牙周炎及侵袭性牙周炎易感性的影响，以期从基因水平探讨牙周炎发病的遗传学机制。
1材料和方法
1.1 研究对象：选择前来深圳市第二中医院口腔科就诊的患者152例。研究对象分成3组：慢性牙周炎患者(57例)、侵袭性牙周炎患者(45例)，牙周健康志愿者(50例)。所有受试者来自深圳市常驻人口，三代均为汉族，社会经济背景相似，无口腔正畸治疗史、无明显错牙合 畸形，无全身系统性疾病。组间无年龄和性别分布差异。慢性牙周炎的纳入标准：全口至少有10颗可进行牙周评价的牙齿,至少有4颗磨牙(不包括第三磨牙)，不同象限至少2个部位牙周袋深度(PD)≥5 mm或附着丧失量(AL)≥2mm，X线显示牙槽骨吸收≥ 1/3。侵袭性牙周炎的纳入标准：快速的附着丧失和骨破坏且与菌斑堆积量不成正比 ......
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