特异引物PCR快速检测可疑牙周致病菌的相关性研究(1)
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[摘要]目的：寻求快速检测伴放线放线杆菌(Aa)、牙龈卟啉菌(Pg)和中间普氏菌(Pi)在牙周感染患者人群中感染率的方法。方法：特异引物PCR，检测3种牙周可疑致病菌在30例中重度牙周病及30名正常组龈沟液中的定植情况。结果：PCR方法检出细菌的最低检测量为3.25ng。Pg在中重度牙周病检出率86.7%，高于正常组13.3%，P<0.001。Pi在中重度牙周病检出率为70%正常组为20%，P<0.001。伴放线放线杆菌在中重度牙周炎Aa检出率为6.7%，正常组检出率为3.3%，P>0.5。结论：PCR方法是特异性强，敏感性高的有效方法。Pg是成人牙周炎可疑致病菌。Pi是成人牙周炎可疑致病菌。Aa可能是牙周正常菌群一部分，需要大样本进一步检测。
[关键词]牙周可疑致病菌；牙周病；PCR
[中图分类号]R783 [文献标识码]A[文章编号]1008-6455(2011)02-0279-03
Study on the specific-primer PCR detection of 3 species of putative periodontal pathogens and the correlation between the presence of bacteria and periodontosis
LI Da-xu1,LI Xiao-hong1,REN Le2,CHANG Xiao-feng1
(1.Department of Oral Impiant,Affiliated Stomatology hospiton ofXi'an JiaotongUniversity,Xi'an 710003,Shaanxi,China;2.Department of Stomatology,The First Affillated Hosptial of collegeof Xi'an Jiaotong University)
Abstract:Objective To establish a rapid multiple specific-primer PCR for detection of three species of bacteria Aa,Pi and Pg,and the correlation between the presence of bacteria and the periodontosis.Methods Subgingival microflora samples were collected from 30 patients with moderate to sever periodotitis and 30 periodontally healthy patients,the PCR was used to amplify the target genes.ResultsSensitivity and specificity of PCR were also evaluated with the reference bacteria. The multiplePCR was able to detect a minimum of 3.25ng bacteria DNA.With good specificity,target fragments of 3 species of bacteria were detected.The prevalence of Pg in adults periodentosis were 86.7% vs 13.3% in the healthy group(P<0.001). The prevalence of Pi in adults periodentosis were 70% vs 20% in the healthy group (P<0.001 ),The prevalence of Aa in adults periodentosis were 6.7% vs 3.3% in the healthy group (P>0.5).Conclusion As the putative periodontal pathogens of adult periodontitis,Pg,Pi,Aamay be part of the normal oral flora,that need futher study.
Key words:putative periodontal pathogens;adult periodotitis;PCR
细菌是牙周病的始动因子,但究竟哪些细菌与牙周病直接相关目前还没有确切的结论。细菌和牙周病临床表现的复杂性及各个实验室方法上的差异都可能导致主要可疑牙周致病菌检出上的差异,目前国外研究检出比较多的有牙龈卟啉菌(Porphyromonasgingivalis,Pg)、中间普氏菌(Prevot ellaintermedia,Pi)、伴放线放线杆菌(A ......
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