血清微环境对小鼠T细胞衰老的调节作用
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[摘要]目的：探讨血清微环境对小鼠T细胞衰老的调节作用。方法：分别取年老(12～14月龄)及年轻(1.5～2月龄)小鼠各10只，提取其脾脏淋巴细胞及血清，实验分4组。组I为年老鼠T淋巴细胞+10%年轻鼠血清；组II为年老鼠T淋巴细胞+10%年老鼠血清；组III为年轻鼠T淋巴细胞+10%年轻鼠血清；组IV为年轻鼠T淋巴细胞+10%年老鼠血清。培养48h后，经流式细胞术研究CD8+CD28+共表达率差异。结果：组I和组II T细胞表面的CD8+CD28+共表达率分别是(10.84±0.6841)%和(3.18±0.1789)%，组III和组IV T细胞表面的CD8+CD28+共表达分别是(12.5±0.9445)%和(8.36±0.2074)%。各组间对比有统计学差异(P<0.05)结论：血清微环境具有调节小鼠T细胞衰老的作用，年轻鼠血清能使年老鼠的T细胞表面的CD8+CD28+共表达率提高，年老鼠的血清能使年轻鼠的T细胞表面的CD8+CD28+共表达率降低。
[关键词]血清微环境；T淋巴细胞；免疫衰老；流式细胞术
[中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2011)06-0946-03
Effects of serum microenvironment on CD8+CD28+T cell
(SHI Bing-bing1,ZHANG Lei1,ZHOU Bi-yu2,CAO Yi-mei2,GAO Feng2,YIN Ning-bei2,YAN Li1, ZHAO Zhen-ming2
(1.Research center,2.The First Department, Plastic Surgery Hospital，Chinese Academic Medical Science and Peking Union Medical College，Beijing 100144，China)
Abstract： ObjectiveTo observe the effects of serum microenvironment on CD8+CD28+T cells. MethodsMouse lymphocytes were isolated from spleen,these cells from young mouse and old mouse were cultured under the following serum microenvironment. All mouse were divided into four groups: the lymphocytes from old mouse cultured with young serum (group I);the lymphocytes from old mouse cultured with old serum (group II),the lymphocytes from young mouse cultured with young serum (group III)，and the lymphocytes from young mouse cultured with old serum (group IV).The co-expression of CD8+CD28+ on T cells was detected by the flow cytometry.ResultsIn the group of lymphocytes from old mouse cultured with young serum, the co-expression of CD8+CD28+ was (10.84±0.6841)%. In the group of lymphocytes from old mouse cultured with old serum group.The co-expression of CD8+CD28+ was (3.18±0.1789)%. In the group of lymphocytes from young mouse cultured with young serum and cultured with old serum group,the co-expression of CD8+CD28+ were (12.5±0.9445 )%and (8.36±0.2074)% , respectively. There were significant statistical differences in these four groups ......
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