富血小板纤维蛋白体外释放VEGF影响因素的探讨(1)
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[摘要]目的：探讨不同材质的离心管和储存温度对制备的富血小板纤维蛋白(platelet-rich fibrin,PRF)释放血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响。方法：分别用玻璃离心管和塑料离心管从健康家兔耳中动脉采血制备PRF，制备成PRF膜片后，加入无菌DMEM培养液各分为两组置于37℃和4℃条件下，并分别从 20min，4h，24h，72h，120h， 168h，0～168h七个时间点收集PRF样本，用ELISA试剂盒检测各时间点VEGF的含量情况。结果：4℃条件下保存的PRF膜片释放VEGF含量高于37℃，玻璃离心管制备的PRF释放的VEGF含量高于塑料离心管。结论：不同的材质离心管和不同的储藏温度对PRF的特性存在一定影响，PRF的制备宜用玻璃离心管，在使用前宜储藏于4℃条件下。
[关键词]PRF；VEGF；温度；材质
[中图分类号]R332 [文献标识码]A [文章编号]1008-6455(2011)03-0427-04
Investigation of production and influential factors of platelet-rich fibrin
LI Long1,ZHAO Jian-hui2,LIU Bin3,LING HU Da-ke1,LIU Yan-pu1,LIANG Li-hua1,YANG Yong1, YANG Tao1
(1.Department of Oral and Maxillofacial Surgery, School of Stomatology,The Fourth Military Medical University,Xi'an 710032,Shaanxi,China; 2.Institute of Plastic Surgery,Xijing Hospital,The Fourth Military Medical University,Xi'an 710032, Shaanxi,China; 3.Department of Oral Biology and Laboratory Animal Center,School of Stomatology,The Fourth Military Medical University,Xi'an 710032,Shaanxi,China)
Abstract: Objective To investigate the influence factors of platelet-rich fibrin (PRF) releasing vascular endothelial growth factor (VEGF) with two different kinds of collection tubes (dry glass tubes and plastic tubes) and with different storage temperature (4 degree and 37degree). Methods PRF was produced with two different kinds of collection tubes(dry glass tubes and plastic tubes) from healthy rabbit's medium-sized artery of the ears, and saved in different storage temperature (4 degree and 37degree ) to test the quantity of released VEGF,and it was tested at seven experimental times: 20 min, 4 h, 24 h (day 1),72h (day 3),120 h (day 5) and 168 h (day 7),0-168h(7days) with ELISA kit. Results The total quantity of VEGF with dry glass tubes was higher than that with plastic tubes, in the temperature group it showed that the total quantity of VEGF saved in 4 degree was higher than in the 37 degree. Conclusion Different kinds of collection tubes and different storage temperatures may affect the characteristics of PRF ......
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