黑木耳多糖对ICR小鼠皮肤光老化的保护作用(1)
 |
| 第1页 |
参见附件。
[摘要]目的：探讨黑木耳多糖(Auricularia auricular polysaccharide,AAP)对ICR小鼠皮肤光老化的保护作用。方法：选取健康雌性ICR小鼠共50只，随机分成5组各10只：正常组(A)、模型对照组(B)、模型+AAP低剂量(50 mg/kg.d)灌胃组(C)、模型+AAP中剂量(100 mg/kg.d)灌胃组(D)、模型+AAP高剂量(200 mg/kg.d)灌胃组(E)。长波紫外线(ultraviolet A, UVA)联合中波紫外线(ultraviolet B,UVB)照射小鼠制备皮肤光老化模型。每周一、三、五照射，第1周每次照射时间1h，以后每周依次增加1h，自第5周起为5h，直至第14周照光结束。每次照光前各治疗组小鼠给予不同浓度的AAP灌胃。实验结束后取小鼠背部皮肤用测试盒测定超氧化物歧化酶(superoxide dismutase,SOD)及谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-PX)活力、丙二醛(malondialdehyde，MDA)含量；组织切片HE染色观察皮肤结构改变。结果：与正常组比较,模型对照组皮肤组织中SOD、GSH-PX活力显著降低(P<0.05)，MDA含量显著增高(P<0.05)；与模型组对照组比较，AAP各灌胃组SOD、GSH-PX活力显著升高(P<0.05)，MDA含量显著降低(P<0.05)。模型对照组皮肤出现皮屑、褐黄、粗糙肥厚、深皱纹、缺乏弹性等光老化特征，组织切片呈现光老化改变。AAP各治疗组皮肤外观及组织学特点均较模型对照组明显改善。结论：AAP能有效提高小鼠抗氧化能力，对小鼠皮肤光老化有保护作用。
[关键词]光老化；黑木耳多糖；ICR小鼠
[中图分类号]R332 R758 [文献标识码]A [文章编号]1008-6455(2012)05-0759-03
Protective effects of Auricularia auricular polysaccharide on skin photoaging in ICR mice
YI Qing-ling,ZENG Wei-hui,LIU Yan-ting,XU-lei
(Department of Dermatology of the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004，Shanxi,China;2.Department of Dermatology of The Second Hospital of Chengdu)
Abstract: Objective To study the protective effects of Auricularia auricular polysaccharide (AAP)on photoaged ICR mice skin. Methods Fifty healthy female ICR mice were divided into five groups (n = 10) randomly: normal group(A)，model control group(B),at dose of 50 mg/kg.d group(C), at dose of 100 mg/kg.d group(D), at dose of 200 mg/kg.d group(E). To establish skin photoaging mice models by irradiated UVA and UVB three times weekly (Monday,Wednesday and Friday).In the first week the time of UV exposure was 1 hour each time, then increased by 1 hour per week until week 5, and maintained the time until the 14th week ......
您现在查看是摘要介绍页，详见PDF附件(2691kb)。