当归水煎液对人脐静脉内皮细胞增殖、凋亡及胶原合成的影响(1)
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[摘要]目的：探讨当归水煎液对人脐静脉内皮细胞增殖、凋亡和胶原合成的影响。方法：体外培养人脐静脉内皮细胞，用噻唑蓝比色法检测细胞增殖活性，用流式细胞术分析细胞周期及凋亡，用放射免疫法测定胶原合成。结果：当归水煎液能促进人脐静脉内皮细胞的增殖，且随浓度增大促增殖作用愈大。当归水煎液使S期和G2/M期细胞增多，G0/G1期细胞减少(P<0.05)，凋亡率逐渐减小(P<0.05)。当归水煎液呈剂量和时间依赖性抑制人脐静脉内皮细胞合成胶原 (P<0.05)。结论：当归水煎液呈剂量依赖性促进人脐静脉内皮细胞的增殖，抑制其凋亡，减少胶原合成。
[关键词]当归水煎液；内皮细胞；增殖；凋亡；胶原合成
[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455(2012)05-0782-03
Effect of angelic decoction on proliferation, apoptosis, collagen synthesis of human umbilical vein endothelial cells
LIU Kai,ZHANG Xuan-fen,ZHANG Jin,QIN Yong-hong,ZHONG Lin
(Plastic Surgery,The Second Hospital of Lanzhou University,Lanzhou 730030,Gansu,China)
Abstract Objective To investigate the effects of angelic decoction on proliferation, cell cycle, apoptosis, and collagen synthesis of human umbilical vein endothelial cells (HUVEC). Methods HUVEC was cultured and passaged in Dulbecco's modified Eagle's medium(DMEM) and treated with Angelicanaphtha 1mg/L, 4mg/L, and 16mg/L at 1, 3, 5, and 7day respectively. The proliferation was measured with MTT method. The cell cycle and apoptosis were analyzed with Flow cytometry and collagen synthesis was determined with radioimmunoassay. Results The HUVEC proliferation was accelerated by Angelic decoction in concentration-dependent manner (P<0.05). S phase cells and G2/M phase cells were increased and G0/G1 phase cells and apoptosis was gradually reduced by Angelic decoction (P<0.05). The collagen synthesis of HUVEC was also inhibited by Angelic decoction in concentration-dependent manner (P<0.05). Conclusion The HUVEC proliferation was accelerated and the apoptosis was reduced and the collagen synthesis was reduced by Angelic decoction.
Key words:angelic decoction; endothelial cells; proliferation; apoptosis; collagen synthesis
创面愈合和瘢痕形成是新血管形成的结果[1]，内皮细胞增殖和合成细胞外基质是血管形成的基础, 脐静脉内皮细胞是调控血管形成的最佳靶点之一[2-3]。研究表明，当归挥发油对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)的增殖呈小剂量刺激、大剂量抑制的双向调节作用，对胶原合成呈抑制效应[4]。然而，当归水煎液对人脐静脉内皮细胞增殖、凋亡和胶原合成的影响未见报道。我们的研究旨在探讨当归水煎液对创面愈合和瘢痕形成的作用。
1 材料和方法
1.1材料
1.1.1 主要试剂：高糖DMEM细胞培养基(Gibco公司) ，胎牛血清(中美合资兰州民海生物工程有限公司) ......
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