细辛脂素联合受体脾细胞诱导心脏移植免疫耐受的研究(1)
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【摘要】目的：本实验通过术前7日和术前3日输注不同剂量供者脾细胞于受体胸腺内，联合中药细辛中的细辛脂素成分，探讨其对心脏移植免疫耐受的诱导，并对诱导效果加以比较。方法：提取中药细辛中的细辛脂素成分。健康雄性Wistar大鼠(供体)和SD大鼠(受体)各80只随机配对，建立大鼠腹部异位心脏移植模型，将接受心脏移植的SD大鼠分成5组：A组：阳性对照组，行腹部异位心脏移植，不给任何处置；B组：细辛脂素组：行腹部异位心脏移植，术前7天开始灌服细辛脂素25 mg·kg-1·d-1；C组：脾细胞诱导组1，术前7天开始输注供者脾细胞于受体胸腺内；D组：脾细胞诱导组2，术前7天开始输注供者脾细胞于受体胸腺内，术前7天开始灌服细辛脂素25 mg·kg-1·d-1；E组：脾细胞诱导组3，术前3天开始输注供者脾细胞于受体胸腺内，术前7天开始灌服细辛脂素25 mg·kg-1·d-1。每组各8只术后第7天处死。另外8只用于观察移植物生存天数。噻唑蓝(MTT)法检测供受体混合淋巴细胞反应；应用ELISA法检测受体血清中IL-2、IFN-γ、IL-4和IL-10水平； 结果：1、免疫诱导组联合细辛脂素组(D组和F组)移植心存活时间较阳性对照组显著延长(P<0.01)。2、供受体混合淋巴细胞反应免疫诱导组较阳性对照组差异有显著性(P<0.01)。3、免疫诱导组联合细辛脂素组(D组和F组)移植物生存时间和外周血细胞因子均有显著性差异(P<0.05)。结 论：1、通过术前7日胸腺内输注脾细胞可以诱导心脏移植免疫耐受，为供者特异性免疫耐受。2、术前7日预处理对心脏移植免疫耐受的诱导较术前3日预处理组理想。
【关键词】细辛脂素;心脏移植;脾细胞;细胞因子;免疫耐受
【中图分类号】R965 【文献标识码】A 【文章编号】1008-6455(2011)12-0019-02
The Induction of Immune Tolerance in Rats Cardiac Allograft by Asarinin Combined with splenocyte
Zhang Lili Wang Xiaoyun Chen Yanjun1 et al.
【Abstract】Objective: To study the effect of immune tolerance in rats cardiac allograft induced by donor splenocyte 7 days and 1day before operation combined with Asarinin. Methods: To extract the asarinin from herba asari. The 80 SD rats (receptor) and 64 wistar rats (donor) were divided into five groups: group A (control group); group B (Asarinin treated 7 days before operation); group C (splenocyte treated 7 days before operation); group D (splenocyte treated 7 days before operation and Asarinin); group E (splenocyte treated 3 days before operation and Asarinin).Some were to examine survival time(n 8) and others were to observe mixed lymphocyte reaction(MLR) between the donor and receptor with MTT; the level of IL-2,IFN-γ,IL-4 and IL-10 in serum with ELISA postoperatively 7 days (n 8). Result: 1.Allografts survival time is prolonged in group D and E (P<0.01). 2.The lymphocyte proliferation is alleviated in group D and E comapred with group A(P<0.01). 3.The level of IL-4 and IL-10 significantly increases in group D and E compared with group A (P<0.01). Conclusion: 1. Immune tolerance can be induced by donor splenocyte 7days before operation and is specific tolerance to donor ......
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