尼古丁对小鼠骨髓细胞微核影响的实验研究(1)
 |
| 第1页 |
参见附件(2838KB，2页)。
【摘要】目的：探讨香烟成分尼古丁对骨髓细胞微核产生的影响。方法：建立小鼠骨髓嗜多染红细胞微核实验模型，将小鼠随机分为尼古丁(低、中、高剂量)实验组、顺铂对照组、乙醇对照组、生理盐水对照组，每组10只，采用腹腔注射给药，油镜下检测骨髓中嗜多红染细胞(PCE)微核情况及绘制量效关系曲线。结果：尼古丁低剂量组和乙醇对照组的微核发生率分别为(1.90±0.88)%和(0.70±0.22)%，两组间有显著差异(P<0.05)。采用不同剂量尼古丁染毒小鼠，其骨髓细胞微核率呈递增趋势。结论：尼古丁在嗜多染红细胞微核实验中具有明显的致突作用。
【关键词】尼古丁；骨髓；嗜多染红细胞；微核；细胞毒性
【中图分类号】R730.55 【文献标识码】A 【文章编号】1008-6455(2011)12-0118-02
Affected on bone marrow erythrocyte micronucleus of mice by Nicotine
Shi Qiang Zheng Yongbin1* Sui Fengying2 et al.
【Abstract】Objective:To investigate the impacts of Nicotine on micronucleus in mouse bone marrow. Methods:Micronucleus test in polychromatic erythrocytes of bone marrow in mice was performed. The mice was divided into six group, there was solution group with injection of nicotine by low doses, middle doses and high doses, and control group with injection of CDDP, ethanol and saline. Ten mice in each group, the drugs were injected into abdominal. Observed micronucleus cells in bone marrow cells under Oil immersion, and drawing quantity-effect relationship curve. Results:There was apparent increasing of micronucleus frequency in nicotine low-doses solution group comparing with ethanol control group (P<0.05), two group of micronucleus rats is (1.90± 0.88) % and (0.70± 0.22) %.For the following poisoning at different doses, the drug had apparent action on increasing micronucleus frequency in mice. Conclusion:In this experiment, it is viewed primarily that cigarettes ingredient of nicotine has significant mutagenic effect on polychromatic erythrocytes micronucleus test.
【Key words】Nicotine; bone marrow; polychromatic erythrocytes; Micronucleus; Cytotoxi
尼古丁是香烟及烟雾中的主要毒物成分，常温下为无色或浅黄色油状液体，极易溶于酒精〖1〗。在烟叶尼古丁含量最高，唯有其会产生耐受、依赖、敏化以及戒断症状〖2〗。目前关于香烟成分尼古丁人体的危害主要从宏观的角度进行阐述，缺乏微观水平的毒性学证据。本实验拟通过尼古丁对小鼠骨髓嗜多染红细胞(Polychromatic erythrocytes，PCE)产生微核的影响进行研究，以期提供尼古丁对人体危害的细胞毒理学证据。
1 材料与方法
1.1 材料
1.1.1 实验动物:健康雄性昆明种小鼠60只，体重25～35 g。由嘉兴学院医学实验中心提供。动物饲养室条件：室温(23 ℃±2 ℃)，湿度50～60%，采用12 h明暗光照，定时通风。所有动物以标准颗粒饲料饲养，自由饮食水。
1.1.2 实验试剂:由市场购得某牌过滤嘴香烟，烤烟型，烟气烟碱量1.12 mg、焦油量17 mg。顺铂(CDDP)购自上海洽姆仪器科技有限公司 ......
您现在查看是摘要介绍页，详见PDF附件(2838KB，2页)。