法测定保济丸中葛根素的含量
关键词:高效液相色谱法;保济丸,葛根素
摘要目的:运用高效液相色谱法测定保济丸中葛根素的含量。 方法: 色谱柱为Hypersil ODS C18柱(250mm×4.6m,m5mm),甲醇-水(25∶75)为流动相,检测波长为250nm。结果:葛根素在0.0325~0.3256mg线性关系良好(r=0.9998,平均回收率为98.12%,RSD2.11%。结论:本法简单,结果可靠,重复性好,将为保济丸质量控制提供科学依据。
KEY WORD: HPLC;Baoji Pills;
ABSTRACT: AIM: To detemine the contents of puerarin in Baoji Pills by HPLC.
METHODS: In this method, Hypersil ODS C18 column was used, and methanol-water(25:75) as a mobile phase, the detection wavelength was at 250nm. RESULTS: Puerarin showed a good linear relationship at a range of 0.0325~0.3256mg ......
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