HPLC法测定亚贡叶中绿原酸的含量
摘 要:目的:建立亚贡叶中绿原酸的含量测定方法。方法:采用高效液相色谱法测定亚贡叶中绿原酸的含量。色谱柱:Agilent Zorbax-C18(250mm×4.6mm,5μm),流动相:乙腈~0.4%磷酸(10:90),流速为1.0mL/min,检测波长:327nm,柱温为25℃。结果:绿原酸含量的线性范围为0.39~1.93μg,r=0.9992,加样回收率为97.5%,RSD为2.0%。结论:测定方法简便、准确、重现性好,适用于亚贡叶的质量控制。
关键词:亚贡叶;HPLC法;绿原酸
中图分类号:R285.5
文献标识码:A
文章编号:1673-7717(2008)03-0580-02
The Content Determination of the Content Chlorogenic Acid in Smallanthus Sonchifolius by HPLC
WEI Qing-rong,DOU De-qiang,TIAN Fang,KANG Ting-guo
(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning China)
Abstract:Objective:To establish an analytical method for determinating chlorogenic acid in Smallanthus sonchifolius. Methods:The determination was measured by HPLC with Agilent Zorbax-C18(250mm×4.6mm ......
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