人参皂苷Rg3联合紫杉醇抗胃癌转移的作用及机制研究(1)
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(浙江省人民医院,浙江 杭州 310014)
摘 要：目的：探讨人参皂苷Rg3联合紫杉醇协同抗胃癌转移的作用及机制。方法：将人胃癌BGC—823细胞裸鼠移植瘤动物模型，随机分为四组(每组10只)：人参皂苷Rg3组[GS-Rg3：10mg/(kg·d)灌胃])，紫杉醇组(10 mg/kg腹腔内注入，每周二次)，联合组(人参皂苷Rg3+紫杉醇，用法同上)，对照组(生理盐水组)。连续用药3周。用药结束后脱颈处死裸鼠，统计各组转移率，免疫组化检测微血管密度(MVD)，RT-PCR测定血管生成因子β3-整合素的mRNA水平。结果：联合组肿瘤转移率低于紫杉醇组和人参皂苷Rg3组(P<0.05， P<0.01)，联合组肿瘤微血管密度(MVD)最低，与人参皂苷Rg3组和紫杉醇组比较有显著差异(P<0.05和P<0.01)，联合组β3-整合素最低，与紫杉醇组、人参皂苷Rg3组比较均有显著差异(P<0.05，P<0.01).结论：人参皂苷Rg3联合紫杉醇具有协同抗胃癌转移作用，可能通过协同抑制肿瘤新生血管形成有关。
关键词：胃癌；转移；人参皂苷Rg3；紫杉醇；β3-整合素
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0625-04
Experiment Study of Gisenoside Rg3 and Paclitaxel on inhibiting Gastric Cancer
Cells’ Metastasis and Its Mechanism
YUAN Guo-rong,YE Zai-yuan
(Zhejiang Provincal People’ Hospital,Hangzhou 310014，Zhejiang,China)
Abstract:Objective:To explore inhibiting Gastric cancer Cells’ Metastasis and Its Mechanism of Gisenoside Rg3 and Paclitaxel. Methods: Transplantation tumor models of gastric cancer cells BGC-823 in SCID mice were randomly divided into four groups(10 mice per group): Ginsenoside Rg3 group(GS-Rg3: 10mg/Kg/d intragastric administration, for three weeks ), Paclitaxel group(Paclitaxel at 10mg/Kg was injected into abdominal cavity two times per week,for three weeks) , the combined group (GS-Rg3+ Paclitaxel, using the same method above). After medication was over, every group of mice were decapitated, cancer metastasis ratesand inhibition rates were tested; immunohistochemistry was to detect microvessel density (MVD); RT-PCR was to detect mRNA expression of β3-integrin.Result: Tumor metastasis rate of the combined group was lower than GS-Rg3 group and Paclitaxel group(P<0.05 ......
(浙江省人民医院,浙江 杭州 310014)
摘 要：目的：探讨人参皂苷Rg3联合紫杉醇协同抗胃癌转移的作用及机制。方法：将人胃癌BGC—823细胞裸鼠移植瘤动物模型，随机分为四组(每组10只)：人参皂苷Rg3组[GS-Rg3：10mg/(kg·d)灌胃])，紫杉醇组(10 mg/kg腹腔内注入，每周二次)，联合组(人参皂苷Rg3+紫杉醇，用法同上)，对照组(生理盐水组)。连续用药3周。用药结束后脱颈处死裸鼠，统计各组转移率，免疫组化检测微血管密度(MVD)，RT-PCR测定血管生成因子β3-整合素的mRNA水平。结果：联合组肿瘤转移率低于紫杉醇组和人参皂苷Rg3组(P<0.05， P<0.01)，联合组肿瘤微血管密度(MVD)最低，与人参皂苷Rg3组和紫杉醇组比较有显著差异(P<0.05和P<0.01)，联合组β3-整合素最低，与紫杉醇组、人参皂苷Rg3组比较均有显著差异(P<0.05，P<0.01).结论：人参皂苷Rg3联合紫杉醇具有协同抗胃癌转移作用，可能通过协同抑制肿瘤新生血管形成有关。
关键词：胃癌；转移；人参皂苷Rg3；紫杉醇；β3-整合素
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0625-04
Experiment Study of Gisenoside Rg3 and Paclitaxel on inhibiting Gastric Cancer
Cells’ Metastasis and Its Mechanism
YUAN Guo-rong,YE Zai-yuan
(Zhejiang Provincal People’ Hospital,Hangzhou 310014，Zhejiang,China)
Abstract:Objective:To explore inhibiting Gastric cancer Cells’ Metastasis and Its Mechanism of Gisenoside Rg3 and Paclitaxel. Methods: Transplantation tumor models of gastric cancer cells BGC-823 in SCID mice were randomly divided into four groups(10 mice per group): Ginsenoside Rg3 group(GS-Rg3: 10mg/Kg/d intragastric administration, for three weeks ), Paclitaxel group(Paclitaxel at 10mg/Kg was injected into abdominal cavity two times per week,for three weeks) , the combined group (GS-Rg3+ Paclitaxel, using the same method above). After medication was over, every group of mice were decapitated, cancer metastasis ratesand inhibition rates were tested; immunohistochemistry was to detect microvessel density (MVD); RT-PCR was to detect mRNA expression of β3-integrin.Result: Tumor metastasis rate of the combined group was lower than GS-Rg3 group and Paclitaxel group(P<0.05 ......
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