姜黄素对实验性变态反应性脑脊髓炎模型的干预研究(1)
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摘 要：目的： 观察姜黄素对实验性变态反应脑脊髓炎大鼠神经行为学及脑组织病理的影响。方法：采用豚鼠脊髓匀浆诱导EAE模型，治疗组给予姜黄素进行干预，观察行为学变化，LFB染色观察脑组织病理改变。结果：与EAE组相比，姜黄素治疗组临床评分明显下降，病程缩短，而且恢复较快。脑组织未见明显炎性细胞浸润，髓鞘脱失略有减轻。正常对照组未见明显异常。结论： 姜黄素能明显改善EAE大鼠临床症状，抑制炎症细胞浸润，促进恢复，对EAE具有一定的治疗作用。
关键词：实验性变态反应性脑脊髓炎；姜黄素；炎症浸润
中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2012)01-0169-03
Intervention Effect of Curcumin on the Model of Experimental Autoimmune Encephalomyelitis
YANG Xuezhi1,WANG Zhaowei1,LI Jianmin1,ZHANG Zhengxue1，YOU Wenting2,ZHU Jiejin2
(1. Department of neurology, The First Affiliated Hospital, Wenzhou Medical College，Wenzhou 325000,Zhejiang,China;
2.School of Pharmacy, Wenzhou Medical College，Wenzhou 325035,Zhejiang,China)
Abstract:Objective:To observe the effect of curcumin on the neuroethology and pathology in brain of rats with experimental autoimmune encephalomyelitis (EAE). Method:The animal model was established in SD rat by injecting guinea pig spinal cord homogenate in complete Freund's adjuvant (CFA) and bordetella pertussis vaccine. Experimental group was given curcumin treatment, then the changes of clinical symptoms were observed every day. Pathological changes of brain were observed by Luxol Fast Blue (LFB) staining. Result:Compared with EAE, the clinical scores and disease course were obviously decreased in curcumin groups, furthermore the rats were recuperated quickly. No obvious infiltration of inflammatory cells in brain but demyelination was a little extenuation. The control group was normal. Conclusion:Curcumin can improve the symptoms of EAE rats, inhibite inflammatory cell infiltration and promote recovery. Thus curcumin has therapeutical effect on EAE.
Key words:experimental autoimmune encephalomyelitis;curcumin;inflammatory infiltration
多发性硬化 (multiple sclerosis，MS)是一种以中枢神经系统白质炎性脱髓鞘病变为特点，主要由CD4+T细胞介导的自身免疫性疾病，确切病因及发病机制尚未阐明。实验性变态反应性脑脊髓炎(experimental autoimmune encephalomyelitis，EAE)由于在临床表现、病理学、免疫学方面与MS有诸多相似之处，是目前公认的用于研究MS发病机制及治疗的理想动物模型。
姜黄素(Curcumin)是从姜科姜黄属植物中提取的一种黄色酸性酚类物质，大量研究表明，姜黄素具有抗氧化、抗炎、抗肿瘤、抗突变及神经保护等药理学作用。近年来国外的实验研究表明，姜黄素对EAE具有保护作用，能够减轻疾病的严重程度及临床症状持续的时间，并能促进其恢复。本实验采用SD大鼠制作的EAE动物模型，通过神经功能缺损评分、病理学方面对姜黄素干预EAE大鼠的疗效进行初步研究，以探讨姜黄素对治疗EAE大鼠的可行性 ......
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