LC-MS/MS法快速筛选保健食品中非法添加的18种止咳平喘化学成分(1)
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摘 要：目的：建立保健食品中非法添加的18种止咳平喘化学成分的快速筛选方法。方法:采用液相色谱-串联质谱法(LC-MS/MS)以Polaris 5 C18-A (2.0mm×50mm，5μm)为分析柱，流动相采用0.1%甲酸和甲醇进行梯度洗脱，电喷雾电离(ESI)、多反应监测(MRM) 扫描方式，对保健食品中非法添加的18种止咳平喘化学成分进行筛选分析。结果:建立了快速分离检测保健食品中18种非法添加成分的测定方法，检测限低于2ng。结论:该方法专属性强、灵敏度高，适用于止咳平喘类保健品中非法添加成分的筛选及确证工作。
关键词：高效液相色谱-质谱；多反应监测；止咳平喘类保健品中非法添加成分；快速
中图分类号：R284.2 文献标识码：A 文章编号：1673-7717(2011)12-0172-04
Screening for 18 Mixed Illegally Cough-Relieving Chemical Composition in Health Food by LC-MS/MS
WANG Zhenyuan1,LI Dan1,WEN Hongmei2,CUI Fuchun1,HUANG Ya1, SUN Xiarong1
(1.Nanjing Institute for Drug Control,Nanjing 210015,Jiangsu,China;
2.Nanjing University of Traditional Chinese Medicine,Nanjing 210029,Jiangsu,China)
Abstract:Objective：To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening 18 kinds of cough-relieving cough asthma chemical compositions in health food.Methods：This method involves liquid chromatography - tandem mass spectrometry,the separation was conducted by Varian Polaris 5 C18-A (2.0mm×50mm,5μm).The mobile phase was consisted of 0.1% formic and methanol.The target compounds were analyzed with LC-MS/MS- ESI,multiple-reaction monitoring(MRM) was used to screen illegal chemical compositions in health food.Results：A fast and sensitive liquid chromatography mass spectrometric (LC/MS) method for the simultaneous screening for illegal chemical compositions was described.The LOD of these substances were below 2ng.Conclusion:The method was sufficiently selective and sensitive to detect illegal chemical compositions in health food.
Key words:LC-MS/MS； multiple- reaction monitoring(MRM)；mixed illegally chemical compositions；rapid
随着国家监管力度的加强，药品市场已经相对比较健康发展，但是保健品食品市场，却存在鱼龙混杂，良莠不齐的问题，虚假广告、违规保健品配方等等让消费者为之困惑。很多保健品食品常被冠以“最新科技成果”或“祖传秘方”[1]，但其具体作用机理却模糊不清；更有不法分子为求速效，在其中添加化学药品[2-5]，消费者在不知情的情况下服用了未知成分未知剂量的药品，极易产生不良后果。目前，国家药品监督管理局对止咳平喘类成分还没有一个比较系统的检验方法，本实验运用液质联用技术，采用多反应监测扫描方式，建立了同时检测非法添加的18种止咳平喘化学成分的快速检验方法，为更好打击不法行为提供可靠的技术保障。
1仪器与试药
1.1仪器
1200L LC-MS液质联用仪(美国瓦里安公司)：配有自动进样器 ......
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