5种中药注射剂对多柔比星所致心肌H9c2损伤保护作用(1)
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摘要：目的：考察5种中药注射剂对多柔比星(DOX)作用下H9c2心肌细胞损伤的保护作用。方法：应用H9c2心肌细胞筛查体系，通过检测细胞存活率、过氧化物岐化酶(SOD)活性和氧化代谢产物(MDA)含量，考察参麦注射剂、丹参注射剂、冠心宁注射剂、香丹注射剂和黄芪注射剂等5种中药注射剂对暴露于1.7×10-4至2.2×10-9 M浓度DOX下H9c2心肌细胞的存活率的影响。结果：黄芪注射剂和香丹注射剂能提高DOX作用下心肌细胞的存活率，有效地缓解DOX所引起的心肌细胞MDA浓度增高，提高SOD酶活性且不影响DOX的体外抗肿瘤活性。结论：黄芪注射剂和香丹注射剂能降低DOX对心肌细胞的毒性作用，可能是通过增加抗氧化物酶活力，减少氧化产物生成，从而减轻氧化应激损伤这些途径实现的。
关键词：多柔比星；H9c2心肌细胞；丹参注射剂；参麦注射剂；冠心宁注射剂；香丹注射剂；黄芪注射剂
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2012)03-0483-03
Protective Effect of Five Injections on Doxorubicin-Induced
Cytotoxicity in Cardiac Myoblasts H9c2
XIN Yanfei,ZHOU Guoliang,HAN Bin,YOU Zhenqiang,XUAN Yaoxian
(Center of Safety Evaluation,Zhejiang Academy of Medical Sciences,Hangzhou 310013,Zhejiang,China)
Abstract:Objective:To test whether five injections can transfer its antioxidant function into the protection of the doxorubicin (DOX)-induced cardiotoxicity in cardiac myoblasts H9c2. Method:H9c2 cells were exposed to DOX and/or five injection. The peak concentration of five injection of protective effect on DOX-induce was evaluated through examination, level of malondialdehyde (MDA) and superoxide dismutase (SOD) activity.The effects of five injections on the cytotoxicity of DOX to H9c2 cells and A549 lung carcinoma cells were studied. Results:Huangqi injection and Xiangdan injection had protective effect on H9c2 cells against DOX and significantly increased SOD activity and decreased the MDA level in H9c2 experimental system damaged by DOX exposure while not attenuate the antitumor activity of DOX on A549 carcinoma cells. Conclusion:Our evidence indicates that Huangqi injection and Xiangdan injection elicited a typical protective effect on DOX-induced cytotoxicity in cardiac myoblasts H9c2 via suppressing oxidative stress.
Key words:doxorubicin; cardiac myoblasts H9c2; Danshen injection; Shenmai injection; Guanxinning injection; Xiangdan injection; Huangqi injection
收稿日期：2011-11-28
基金项目：国家自然科学基金资助项目(30701064)；国家“十二五”科技重大专项资助项目(2011ZX09301-003)；浙江省科技计划项目(2010F10026，2009C13024)；浙江省中医药管理局资助项目(2007CB187)
作者简介：辛艳飞(1976-)，男，山西人，副研究员，博士，研究方向：药物毒理学。
通讯作者：宣尧仙(1954-)，女，浙江人，研究员，研究方向：药物毒理学。多柔比星(Doxorubicin，DOX)是一种广谱高效的蒽环类抗肿瘤药物，但其所引起心肌损伤和充血性心力衰竭是DOX临床应用的主要不良反应之一[1-2] ......
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