莪术油对大鼠乳腺癌癌前病变组织中VEGFmRNA表达的影响(1)
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摘 要：目的：通过观察莪术油对大鼠乳腺癌癌前病变组织中血管内皮生长因子(VEGF)及其mRNA表达的影响，探讨莪术油治疗乳腺癌前病变的机理。方法：275只SD大鼠随机分为空白对照组、疾病模型组、三苯氧胺组、康莱特组及莪术油小、中、大剂量组。采用DMBA诱导乳腺癌癌前病变造模，干预治疗4周，第8～14周分4批处死动物，采用原位杂交法测定标本中VEGF mRNA表达情况。结果：各试验组不同类型乳腺组织中VEGF mRNA表达阳性率及阳性细胞阳性率呈递增趋势。非典型增生乳腺组织中，各干预组VEGF mRNA表达阳性率均明显低于造模对照组(P<0.05)。各干预组的VEGF mRNA阳性细胞阳性率均明显低于造模对照组(P<0.05)，莪术油组低于康莱特和三苯氧胺组(P<0.05)。对VEGF mRNA表达阳性的非典型增生标本，莪术油大剂量组VEGF mRNA阳性细胞阳性率明显低于小剂量组(P<0.05)。结论：莪术油能够有效的降低DMBA诱导大鼠乳腺癌前病变组织中VEGFmRNA表达强度，抑制血管生成，可能是其阻断乳腺癌发生的有效机制。
关键词：乳腺癌；癌前病变；莪术油；VEGF；原位杂交
中图分类号：R285.5
文献标识码：A
文章编号：1673-7717(2012)04-0679-03
Zedoary turmeric oil effect on rats with precancerous lesions of breast carcinoma in VEGF mRNA expression
SONG Ai-li, XU Zhen-guo
(Shandong Provincial Traditional Chinese Medical Hospital，Jinan 250014，Shandong，China)
Abstract:Objective:Through observing the influence of zedoary turmeric oil on rats with precancerous lesions of breast carcinoma tissue in vascular endothelial growth factor ( VEGF ) and its mRNA expression, discuss the mechanism of Zedoary turmeric oil treating precancerous lesions of breast cancer.Method：275 SD rats were randomly divided into control group, model group, the TAM group, injection group and zedoary turmeric oil, in small, high dose group,then use DMBA induced the model of precancerous lesions of breast cancer.After intervention treatment for 4 weeks, the animals were sacrificed in 4 batches after 8-14 weeks,using insitu method for the determination of specimens of VEGF mRNA expression.Result：Each test group of different types of breast tissue in VEGF mRNA expression positive rate and positive cells positive rate increased，Atypical hyperplasia of breast tissue，The VEGF mRNA expression positive rate in the intervention group were significantly lower than those in model group，and the intervention group VEGF mRNA positive cells positive rate were significantly lower than those in model group，Zedoary turmeric oil group is lower than injection and TAM group ，In the atypical hyperplasia specimens of VEGF mRNA expression，VEGF mRNA positive cells positive rate of Zedoary turmeric oil the in the large dose group was significantly lower than small dose group.Conclusion: Zedoary turmeric oil can effectively reduce DMBA induced rat mammary carcinoma precancerous lesion tissue VEGFmRNA expression intensity and Inhibit angiogenesis ......
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